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Updated: Oct 16, 2025

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana
Published on: May 21, 2019
Reactive oxygen species and nitric oxide as mediators in plant hypersensitive response and stomatal closure
1Department of Plant Pathology, College of Plant Protection, Anhui Agricultural University, Anhui Province Key Laboratory of Crop Integrated Pest Management, Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes, School of Plant Protection, Hefei, Anhui, China.
Abstract:
Nitric oxide (NO) and reactive oxygen species (ROS) have attracted considerable interest from plant pathologists since they regulate plant defenses via the hypersensitive response (HR) and stomatal closure. Here, we introduce the regulatory mechanisms of NO and ROS bursts and discuss the role of such bursts in HR and stomatal closure. It showed that epidermal sections of leaves respond to pathogens by the rapid and intense production of intracellular ROS and NO. Oxidative stress and H2O2 induce stomatal closure. Catalase and peroxidase-deficient plants are also hyperresponsive to pathogen invasion, suggesting a role for H2O2 in HR-mediated cell death. The analysis reveals that ROS and NO play important roles in stomatal closure and HR that involves multiple pathways. Therefore, multi-disciplinary and multi-omics combined analysis is crucial to the advancement of ROS and NO research and their role in plant defense mechanism.
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