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Fluorescent Sensor Array for Quantitative Determination of Saccharides.

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  • 1Department of Chemistry, Bowling Green State University, Bowling Green, Ohio 43403, United States.

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|October 20, 2021
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Summary

New FRET-based dual chromophore sensors enable accurate, noninvasive monitoring of sugar levels in urine. These fluorescent sensors correctly classify nine different saccharides and quantify glucose in complex samples without pretreatment.

Keywords:
dual chromophore sensor systemsfluorescencesaccharide sensingsensor arrayurine analysis

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Area of Science:

  • Biochemistry and Analytical Chemistry
  • Development of novel fluorescent sensors for saccharide detection.

Background:

  • Accurate sugar level monitoring is critical in diverse fields, including the food industry and human health.
  • Noninvasive methods for monitoring sugars in biological fluids like urine are highly desirable.

Purpose of the Study:

  • To develop and validate FRET-based dual chromophore sensors for saccharide detection.
  • To assess the sensors' ability to classify and quantify various saccharides, including glucose, in complex biological samples.

Main Methods:

  • Utilized Förster Resonance Energy Transfer (FRET) principles with dual chromophore systems.
  • Employed fluorescence spectroscopy to study saccharide-binding properties.
  • Applied sensors for high-throughput saccharide determination and quantitative analysis.

Main Results:

  • Developed two fluorescent sensors capable of 100% correct classification of nine different monosaccharides and disaccharides.
  • Successfully quantified glucose in the presence of competing saccharides (fructose) and in urine without sample pretreatment.
  • Demonstrated a glucose quantification range of 0-60 mM, aligning with standard urinalysis test strips.

Conclusions:

  • The developed FRET-based dual chromophore sensors offer a robust and accurate method for noninvasive sugar level monitoring.
  • These sensors show significant potential for applications in diagnostics and high-throughput screening of carbohydrates in biological fluids.