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Selective Culture Medium for Screening of Fosfomycin Resistance in Enterobacterales
Patrice Nordmann1,2,3,4, Mustafa Sadek1,2,5, José Manuel Ortiz de la Rosa1,2
1Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourggrid.8534.a, Fribourg, Switzerland.
Abstract:
A selective medium for screening fosfomycin (FOS)-resistant Enterobacterales was developed. Performances of this medium were first evaluated by using cultures of a collection of 84 enterobacterial clinical strains (42 FOS susceptible and 42 FOS resistant). The SuperFOS medium showed excellent sensitivity and specificity of detection (100%) in those conditions. Then, by testing spiked stool and spiked urine specimens, it revealed excellent performances, with lower limits of identification ranging from 101 to 102 CFU/ml. This screening medium allows easy and accurate detection of FOS-resistant isolates regardless of their resistance mechanisms.
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