Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Oct 16, 2025

Experimental Assessment of Mouse Sociability Using an Automated Image Processing Approach
08:24

Experimental Assessment of Mouse Sociability Using an Automated Image Processing Approach

Published on: May 15, 2016

8.7K

Increased Sociability in Mice Lacking Intergenic Dlx Enhancers.

Siavash Fazel Darbandi1, Crystal Esau1, Cindy Lesage-Pelletier1

  • 1Department of Biology, University of Ottawa, Ottawa, ON, Canada.

Frontiers in Neuroscience
|October 21, 2021
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antidiabetic Drug Use and Expenditure Trends After National Volume-Based Procurement Policy in Nanjing City: An Interrupted Time-Series Analysis.

Tropical medicine & international health : TM & IH·2026
Same author

Divergent impacts of additives on biological risks associated with antibiotic resistance genes during black soldier fly manure composting: A comparative study of biochar, humic acid, and tea residue amendments.

Journal of hazardous materials·2026
Same author

Pituitary adenomas associated with hydrocephalus: clinical characteristics, risk stratification, and clinical management.

Journal of neuro-oncology·2026
Same author

Humification and greenhouse gas emissions divergently regulated during black soldier fly manure composting system by biochar, humic acid, and tea residue.

Bioresource technology·2026
Same author

Novel biallelic FSIP2 variants cause male infertility with multiple morphological abnormalities of sperm flagella in humans.

Asian journal of andrology·2026
Same author

[Effect of electroacupuncture on intestinal function after laparoscopic colorectal cancer surgery].

Zhongguo zhen jiu = Chinese acupuncture & moxibustion·2026

Deleting Dlx intergenic enhancers in mice impairs gene expression and GABAergic cells, leading to increased sociability and learning deficits, impacting forebrain development.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Genetics

Background:

  • Dlx homeodomain transcription factors are crucial for GABAergic interneuron development.
  • Dlx genes are organized in bigene clusters with intergenic cis-regulatory elements (CREs).
  • Specific CREs like I56i and I12b are active in the developing forebrain.

Purpose of the Study:

  • To investigate the regulatory roles of Dlx intergenic CREs on forebrain development.
  • To understand the impact of Dlx gene regulation on neuronal development and behavior.

Main Methods:

  • Generation of mutant mouse lines with deleted Dlx forebrain intergenic enhancers (I56i-/- , I12b-/-).
  • Analysis of Dlx gene expression and downstream targets (Gad2, Evf2).
  • Assessment of GABA+ cell populations and behavioral tests (sociability, fear conditioning).
Keywords:
DlxGABAWilliams–Beuren syndromeautismdevelopmentgene regulation

More Related Videos

Assessment of Social Interaction Behaviors
06:41

Assessment of Social Interaction Behaviors

Published on: February 25, 2011

94.1K
Assessing Social Dominance in Mouse Models Using the Tube Test
03:34

Assessing Social Dominance in Mouse Models Using the Tube Test

Published on: June 6, 2025

571

Related Experiment Videos

Last Updated: Oct 16, 2025

Experimental Assessment of Mouse Sociability Using an Automated Image Processing Approach
08:24

Experimental Assessment of Mouse Sociability Using an Automated Image Processing Approach

Published on: May 15, 2016

8.7K
Assessment of Social Interaction Behaviors
06:41

Assessment of Social Interaction Behaviors

Published on: February 25, 2011

94.1K
Assessing Social Dominance in Mouse Models Using the Tube Test
03:34

Assessing Social Dominance in Mouse Models Using the Tube Test

Published on: June 6, 2025

571

Main Results:

  • Loss of intergenic enhancers impaired Dlx gene expression and downstream targets.
  • Deletion of I56i caused a transient decrease in developing forebrain GABA+ cells.
  • Mutant mice exhibited increased sociability and learning deficits.

Conclusions:

  • Dlx intergenic enhancers are essential for proper Dlx gene regulation in forebrain development.
  • These enhancers influence GABAergic interneuron development and associated behaviors.
  • Understanding these regulatory elements provides insights into forebrain development and neurological disorders.