Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

3.5K
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
3.5K
Complement System01:27

Complement System

3.8K
The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a...
3.8K
Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

3.4K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.4K
Role of Hematopoietic Growth Factors01:28

Role of Hematopoietic Growth Factors

1.9K
Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...
1.9K
Antimicrobial Proteins01:23

Antimicrobial Proteins

9.8K
Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
9.8K
Classification of Leukocytes01:30

Classification of Leukocytes

3.8K
Leukocytes are classified into two groups based on the presence or absence of cytoplasmic granules. Granular leukocytes, which contain granules, belong to the myeloid lineage and are divided into three subtypes: neutrophils, eosinophils, and basophils. These cells are roughly spherical and characterized by the granules in their cytoplasm.
Neutrophils are the most abundant type of granular leukocytes, comprising 50-70% of all leukocytes. They feature small, evenly distributed granules and a...
3.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Factor H-related proteins and their associated disorders.

The Journal of allergy and clinical immunology·2025
Same author

Autoantibodies Against Factor B and Factor H Without Pathogenic Effects in a Patient with Immune Complex-Mediated Membranoproliferative Glomerulonephritis.

Biomedicines·2025
Same author

Development and Characterization of Novel ELISAs for the Specific Quantification of the Factor H-Related Proteins 2, 3, 4, and 5.

Journal of innate immunity·2025
Same author

Decreased complement 4 and interleukin-10 as biomarkers in aqueous humour for non-exudative age-related macular degeneration: a case control study.

Journal of translational medicine·2025
Same author

SARS-CoV-2 Nucleocapsid Protein Is Not Responsible for Over-Activation of Complement Lectin Pathway.

International journal of molecular sciences·2024
Same author

The human factor H protein family - an update.

Frontiers in immunology·2024

Related Experiment Video

Updated: Oct 16, 2025

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

10.1K

Complement Factor H Family Proteins Modulate Monocyte and Neutrophil Granulocyte Functions.

Éva Kárpáti1, Mariann Kremlitzka1, Noémi Sándor1,2

  • 1Department of Immunology, ELTE Eötvös Loránd University, Budapest, Hungary.

Frontiers in Immunology
|October 21, 2021
PubMed
Summary
This summary is machine-generated.

Factor H (FH) and its related proteins bind to immune cells, influencing their functions beyond complement regulation. These findings offer new insights into innate immunity and potential vaccine development strategies.

Keywords:
complementcytokineextracellular DNAfactor H (FH)factor H-related protein (FHR)monocyteneutrophil extracellular trap (NET)neutrophil granulocyte

More Related Videos

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
11:48

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

11.6K
Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments
07:28

Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments

Published on: July 10, 2013

62.8K

Related Experiment Videos

Last Updated: Oct 16, 2025

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

10.1K
Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
11:48

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

11.6K
Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments
07:28

Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments

Published on: July 10, 2013

62.8K

Area of Science:

  • Immunology
  • Complement System Biology

Background:

  • The complement system is crucial for innate immunity.
  • Factor H (FH) primarily regulates the alternative pathway.
  • Emerging evidence highlights non-canonical roles of FH beyond complement modulation.

Purpose of the Study:

  • To investigate the effects of FH, FH-like protein 1 (FHL-1), FH-related (FHR) proteins (FHR-1, FHR-5), and mini-FH on monocytes and neutrophils.
  • To explore the non-canonical functions of FH family proteins on innate immune cells.

Main Methods:

  • Assessing the binding of FH family proteins to monocytes and neutrophils.
  • Evaluating the impact of these proteins on neutrophil functions like NET formation, adherence, and IL-8 production.
  • Analyzing monocyte adherence and the secretion of inflammatory cytokines (IL-1β, TNFα, IL-10) in response to FH family proteins.

Main Results:

  • FH, FHL-1, FHR-1, FHR-5, and mini-FH bind to both monocytes and neutrophils.
  • FH and FHR-1 modulated neutrophil functions, including NET formation and IL-8 production.
  • FH, mini-FH, and FHL-1 affected monocyte adherence and cytokine secretion, with varying effects observed for FHR-1 and FHR-5.

Conclusions:

  • FH family members exhibit heterogeneous effects on monocyte and neutrophil functions.
  • These non-canonical functions impact key aspects of pathogen killing.
  • FH-based complement inhibitors like mini-FH may possess additional biological activities beyond complement inhibition, relevant for infection protection and vaccine development.