Genome engineering in rodents - status quo and perspectives

Area of Science:

• Genetics
• Molecular Biology
• Biotechnology

Background:

• The CRISPR-Cas9 system, introduced in 2013, has profoundly impacted experimental genetics.
• This technology enables precise manipulation of DNA sequences in various organisms.

Purpose of the Study:

• To provide an overview of CRISPR-Cas9 applications in mouse and rat genetics.
• To discuss germline and somatic cell gene editing strategies.
• To explore future perspectives in genome engineering.

Main Methods:

• Review of CRISPR-Cas9 applications in germline gene editing.
• Analysis of CRISPR-Cas9 applications in somatic cell gene editing and beyond.
• Discussion of emerging CRISPR technologies for large-scale genome engineering.

Main Results:

• CRISPR-Cas9 has revolutionized genetic research in rodents.
• The system facilitates both germline and somatic cell modifications.
• New CRISPR technologies are expanding the possibilities for genome engineering.

Conclusions:

• CRISPR-Cas9 is a powerful tool for genetic manipulation in mice and rats.
• The versatility of CRISPR extends to various editing applications.
• Future CRISPR advancements will enable more extensive genome engineering.