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Related Experiment Videos

Improved procedure for platelet freezing.

G Dayian, H L Harris, G D Vlahides

    Vox Sanguinis
    |January 1, 1986
    PubMed
    Summary
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    New methods prevent platelet aggregation after freezing, improving serotonin uptake and function. This advance supports patients with low platelet counts, like those undergoing leukemia treatment.

    Area of Science:

    • Hematology
    • Biotechnology
    • Cryobiology

    Background:

    • Platelet cryopreservation is crucial for transfusion medicine.
    • Traditional methods using plasma diluents often lead to platelet aggregation upon thawing.
    • Aggregation compromises platelet function and therapeutic efficacy.

    Purpose of the Study:

    • To develop an improved method for platelet cryopreservation.
    • To eliminate aggregation of frozen platelets after thawing.
    • To assess the functional recovery and hemostatic effectiveness of cryopreserved platelets.

    Main Methods:

    • Platelets were frozen using a nonplasma diluent at pH 6.5 in a novel freezing bag.
    • Platelet aggregation was induced by adenosine diphosphate (ADP), epinephrine, collagen, and thrombin.

    Related Experiment Videos

  • Carbon-14-labeled serotonin (14C-serotonin) uptake was measured.
  • Hemostatic effectiveness was evaluated in a thrombocytopenic patient.

    • Main Results:

    • The new method eliminated aggregation of platelets upon thawing or reconstitution.
    • Cryopreserved platelets exhibited improved 14C-serotonin uptake.
    • Platelets demonstrated aggregation responses to various agonists.
    • Maximum 14C-serotonin uptake was observed at thrombocrits up to 15%.
    • Successful hemostatic support was achieved in a patient with acute myelogenous leukemia.

    Conclusions:

    • A nonplasma diluent and new freezing bag effectively prevent platelet aggregation post-thaw.
    • This cryopreservation technique preserves platelet function, including serotonin uptake and aggregation.
    • Autologous frozen platelet transfusions can provide effective hemostatic support in severely thrombocytopenic patients.