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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
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Circular RNA Circ-0003006 Promotes Hepatocellular Carcinoma Proliferation and Metastasis Through Sponging miR-542-3p

Qiang Tu1,2, Xiaoxiang You1,2, Jun He1,2

  • 1Department of Hepatobiliary Oncology Surgery, Jiangxi Cancer Hospital of Nanchang University, Nanchang, People's Republic of China.

Cancer Management and Research
|October 22, 2021
PubMed

Insights

Circular RNA circ-0003006 promotes hepatocellular carcinoma (HCC) progression by sponging miR-542-3p, leading to increased HIF-1A levels. Silencing circ-0003006 inhibits HCC cell growth both in vitro and in vivo.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Hepatocellular carcinoma (HCC) is a prevalent gastrointestinal cancer with unclear underlying mechanisms.
  • Circular RNAs (circRNAs) are increasingly recognized for their roles in various biological functions and disease progression.

Purpose of the Study:

  • To investigate the role and mechanism of circ-0003006 in hepatocellular carcinoma progression.

Main Methods:

  • Bioinformatic analysis identified differentially expressed circRNAs in HCC tissues.
  • Real-time quantitative PCR (RT-qPCR) and fluorescence in situ hybridization (FISH) were used for verification and localization.
  • Cell proliferation, migration, and invasion assays, along with in vivo tumor formation experiments, were conducted.
  • Mechanism was elucidated using RNA pull-down, dual-luciferase reporter assays, and gene silencing/overexpression studies.

Main Results:

  • Circ-0003006 was found to be upregulated in HCC tissues.
  • Knockdown of circ-0003006 significantly inhibited HCC cell proliferation and metastasis in vitro and tumor growth in vivo.
  • Circ-0003006 acts as a molecular sponge for miR-542-3p, thereby upregulating its target gene, HIF-1A.
  • Overexpression of HIF-1A reversed the inhibitory effects of circ-0003006 knockdown.

Conclusions:

  • Circ-0003006 promotes HCC progression both in vitro and in vivo.
  • The mechanism involves circ-0003006 sponging miR-542-3p to release the inhibition on HIF-1A.
Abstract

Keywords:
HIF-1Acirc-0003006circular RNAhepatocellular carcinomamiR-542-3p

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