Related Experiment Video
Updated: Oct 16, 2025

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
The complete chloroplast genome and phylogenetic analysis of Primula stenocalyx Maxim
Yupeng Guo1, Li Ma1, Junqiao Li1
1Qinghai Provincial Key Laboratory of High Value Utilization of Characteristic Economic Plants, The College of Ecological Environment and Resources, Qinghai Minzu University, Xining, Qinghai, P. R. China.
Abstract:
Primula stenocalyx Maxim. is a perennial herb with purple umbel flowers. This alpine plant can survive at altitudes of 2700-4300 m. To explore the chloroplast genome, total DNA was extracted from a sample and sequenced, the reads of the chloroplast genome were assembled and annotated. The chloroplast genome of this plant has a circular form with a length of 153,678 bp comprising a large single-copy region (LSC, 84,236 bp), a small single-copy region (SSC, 17,746 bp), and two inverted repeats (IRs, 25,848 bp). The genome has a GC content of 37%. A total of 133 genes were predicted to contain 88 encoded proteins, 8 rRNAs and 37 tRNAs. The evolutionary history indicates that P. stenocalyx was grouped within Primula and formed a clade with Primula knuthiana with a 100% bootstrap support value. The complete cp genome of P. stenocalyx can serve as a reference for future studies on molecular biology, evolution, population genetics, taxonomy and resource protection.
Related Concept Videos
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
Anatomy of Chloroplasts
The Anatomy of Chloroplasts
Structure of...
Evolutionary Relationships through Genome Comparisons
Export of Mitochondrial and Chloroplast Genes
Protein Transport to the Inner Chloroplast Membrane

