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"Fasting: An Effective Preconditioning Method to Increase Fat Graft Survival".
Han Gyu Cha1, Dong Gyu Kim1, Jiyeon Chang2,3
1Department of Plastic and Reconstructive Surgery, Soonchunhyang University Bucheon Hospital, Soonchunhyang University College of Medicine, 170 Jomaru-ro, Bucheon-si, Gyeonggi-do, Korea.
Aesthetic Plastic Surgery
|October 22, 2021
Summary
Fasting for 24 hours before fat grafting enhances fat graft survival. This preconditioning method improves fat graft volume and weight by promoting lipolysis and angiogenesis.
Area of Science:
- Regenerative Medicine
- Adipose Tissue Biology
- Surgical Innovation
Background:
- Current preconditioning methods for fat grafting often require external manipulation.
- Nutrition significantly influences the balance between lipogenesis and lipolysis.
- Investigating nutritional interventions like fasting could offer a novel approach to enhance fat graft outcomes.
Purpose of the Study:
- To investigate the effect of fasting as a preconditioning strategy before autologous fat grafting.
- To determine if fasting influences lipolysis, adipocyte size, and angiogenesis in adipose tissue.
- To evaluate the impact of fasting on fat graft survival rates.
Main Methods:
- C57BL/6 mice were divided into 24-hour starved and fed groups.
- Analysis of lipolysis, adipogenesis, and angiogenesis-related gene expression in adipose tissue.
- Assessment of grafted fat volume and weight using micro-computed tomography.
- Immunohistochemistry and mRNA analysis to evaluate graft survival.
Main Results:
- Fasting reduced adipocyte size by increasing lipolysis.
- Downregulation of adipogenesis genes and upregulation of lipolysis and angiogenesis genes were observed in starved adipose tissue.
- Fat grafts from starved mice showed approximately 20% greater volume and weight, with increased viable adipocytes and vessels.
Conclusions:
- Fasting for 24 hours before fat harvesting improves fat graft retention volume.
- This improvement is attributed to enhanced angiogenesis via vascular endothelial growth factor (VEGF) induction.
- Fasting presents a novel and effective preconditioning strategy to improve autologous fat graft survival.
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