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Rapid COVID-19 Molecular Diagnostic System Using Virus Enrichment Platform
Yoon Ok Jang1, Hyo Joo Lee1,2, Bonhan Koo1
1Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea.
Biosensors
|October 22, 2021
Summary
A new COVID-19 diagnostic system uses a self-powered assay for rapid RNA extraction and loop-mediated isothermal amplification (LAMP) for sensitive, naked-eye detection of SARS-CoV-2 within 60 minutes.
Area of Science:
- Molecular diagnostics
- Virology
- Biotechnology
Background:
- The COVID-19 pandemic necessitates rapid, accurate diagnostic tests for SARS-CoV-2 to control spread and manage healthcare burdens.
- Existing nucleic acid amplification methods for SARS-CoV-2 detection often lack efficient, rapid sample preparation protocols.
- There is a critical need for simplified, instrument-free diagnostic solutions for widespread accessibility.
Purpose of the Study:
- To develop and validate a rapid molecular diagnostic system for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
- To integrate a self-powered sample preparation assay with loop-mediated isothermal amplification (LAMP) for efficient RNA extraction and detection.
- To enable sensitive, naked-eye detection of SARS-CoV-2 within 60 minutes for potential pre-screening applications.
Main Methods:
- A novel self-powered sample preparation assay utilizing a hydrophilic polyvinylidene fluoride filter and dimethyl pimelimidate for virus enrichment and RNA extraction.
- Integration of the sample preparation assay with a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method.
- Development of a naked-eye detection readout for SARS-CoV-2, adaptable for lateral flow assays.
Main Results:
- The integrated system successfully enriches virus, extracts target RNA, and amplifies it for detection.
- The diagnostic system can detect SARS-CoV-2 within 60 minutes, offering a rapid turnaround time.
- Validation using 23 clinical nasopharyngeal specimens demonstrated the system's accuracy.
Conclusions:
- The proposed COVID-19 molecular diagnostic system offers a rapid, sensitive, and instrument-free method for SARS-CoV-2 detection.
- The self-powered sample preparation combined with RT-LAMP and naked-eye detection simplifies the diagnostic workflow.
- This system has the potential for facile, efficient, and inexpensive point-of-care or home-based pre-screening of COVID-19.
