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Synthesis and Characterization of 2-Decenoic Acid Modified Chitosan for Infection Prevention and Tissue Engineering
Carlos Montez Wells1, Emily Carol Coleman1, Rabeta Yeasmin1
1Department of Biomedical Engineering, The University of Memphis, Memphis, TN 38152, USA.
Marine Drugs
|October 22, 2021
Summary
Modified chitosan nanofibers offer enhanced biofilm resistance and support cell growth, making them promising for wound healing and tissue regeneration applications.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Tissue Engineering
Background:
- Chitosan nanofibers are functional antimicrobial materials.
- Stabilizing nanofibers in aqueous solutions is crucial for applications.
- Antimicrobial fatty acid modification can enhance tissue regeneration.
Purpose of the Study:
- To investigate the acylation of chitosan membranes with 2-decenoic acid for enhanced antimicrobial and antibiofilm properties.
- To characterize the physicochemical properties of the modified membranes.
- To evaluate the cytocompatibility and antibiofilm efficacy of the acylated chitosan membranes.
Main Methods:
- Electrospun chitosan membranes were acylated with synthesized 2-decenoyl chloride.
- Physicochemical properties were analyzed using SEM, FTIR, contact angle, and TGA.
- Antibiofilm activity against S. aureus and P. aeruginosa and cytocompatibility with NIH3T3 fibroblasts were assessed.
Main Results:
- Acylation stabilized chitosan nanofibers in aqueous media, increasing hydrophobicity.
- Modified membranes exhibited reduced S. aureus and P. aeruginosa biofilm formation.
- Fibroblast growth was supported on the acylated chitosan membranes.
Conclusions:
- Acylated chitosan membranes demonstrate enhanced stability, hydrophobicity, and antibiofilm activity.
- These membranes are cytocompatible and support fibroblast growth.
- Acylated chitosan membranes show potential for wound dressings, regeneration scaffolds, and drug delivery systems.
