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Updated: Oct 16, 2025

Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain
Published on: May 20, 2022
Investigating Adult Stem Cells Through Lineage analyses
1Department of Biological Sciences, Columbia University, 1212 Amsterdam Avenue, New York, NY, 10027, USA. ddk1@columbia.edu.
Abstract:
A simple, universal and fundamental definition of adult stem cell communities is proposed. Key principles of cell lineage methods for defining adult stem cell numbers, locations and behaviors are critically evaluated, emphasizing the imperatives of capturing the full spectrum of individual stem cell behaviors, examining a variety of experimental time periods and avoiding unwarranted assumptions. The focus is first on defining fundamentals and then addresses stem cell heterogeneity, potential hierarchies and how individual cells serve the function of a stem cell community.
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Adult stem cells are tissue-specific; hence, they divide to develop the tissue from which they originate. One type of adult stem cell is the epithelial stem cell, which gives rise to the keratinocytes in the multiple layers of epithelial cells in the epidermis of the skin. Adult bone marrow has three distinct types of stem cells:...

