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Updated: Oct 16, 2025

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[Structural disorder within the replicative complex of measles virus: functional implications].

J-M Bourhis1, B Canard1, S Longhi1

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Summary

Measles virus nucleoprotein (N) and phosphoprotein (P) exhibit significant structural disorder. This flexibility is crucial for forming essential replication complexes in Mononegavirales, impacting viral RNA synthesis.

Keywords:
P and N structureParamyxoviridaeinduced foldingintrinsic or structural disordermeasles virusnegative strand RNA virusesreplicationreplicative complex

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Area of Science:

  • Virology
  • Molecular Biology
  • Structural Biology

Context:

  • Measles virus, a member of the Paramyxoviridae family, possesses a negative-sense RNA genome.
  • The viral ribonucleoprotein complex, formed by the nucleoprotein (N) and genome, is central to transcription and replication.
  • The RNA-dependent RNA polymerase (L) interacts with the nucleocapsid via the phosphoprotein (P).

Purpose:

  • To review experimental evidence for structural disorder in measles virus N and P proteins.
  • To explore the prevalence of structural disorder in Mononegavirales replicative complexes.
  • To discuss the functional significance and propose a model for disordered protein domains in viral replication.

Summary:

  • Abundant experimental data indicate significant intrinsic structural disorder in measles virus nucleoprotein (N) and phosphoprotein (P).
  • Structural disorder is a conserved feature across the replicative complexes of Paramyxoviridae and Mononegavirales.
  • A model is proposed where the dynamic flexibility of disordered N and P domains facilitates the formation of a tripartite (N-P-L) complex during replication.

Impact:

  • Highlights the role of intrinsically disordered proteins in viral replication mechanisms.
  • Provides insights into the structural dynamics governing the assembly and function of the viral RNA synthesis machinery.
  • Suggests a mechanism for efficient N protein delivery to nascent RNA during replication, potentially influencing viral propagation.