Related Experiment Video
Updated: Oct 16, 2025

Methods for Studying Uterine Contributions to Pregnancy Establishment in an Ovariectomized Mouse Model
Published on: April 7, 2023
Comparison of 5 Versus 7-Day Ovsynch + Progesterone Releasing Intravaginal Device Protocols (PRID) and a Modified G7G
Christos Brozos1, Evangelos Kiossis1, Savvas Hatzieffraimidis1
1Clinic of Farm Animals, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, 54627 Thessaloniki, Greece.
Abstract:
The aim of this study was to evaluate the efficacy of two timed-AI (TAI) protocols (Group G5D, GnRH and PRID -5d- PGF2a -1d- PGF2a -1d- GnRH, n = 105 and Group G7D, GnRH and PRID-7d- PGF2a -1d- PGF2a -1d- GnRH, n = 98) and a modified G7G protocol combining heat detection (HD) and AI or TAI if HD failed (Group HD, GnRH and PRID -7d- PGF2a -1d- PGF2a -5d- HD or 5d TAI if no HD, n = 92). Pregnancy per AI (P/AI) did not differ between G5D and G7D protocol (G5D: 33.8% vs. G7D: 35.2%, P = 0.85). Cows assigned to G5D and G7D group were pooled as TAI group (GTAI) and further compared to GHD. Within the GHD, more primiparous cows exhibited estrous signs compared to multiparous cows (70.4% vs. 46.2%, P = 0.03). Furthermore, 49 cows (53.3%) were served after HD, whereas 43 cows (46.7%) were served after TAI. There was no difference in P/AI between cows served after HD (51.6%) or after TAI (43.0%, P = 0.49). GHD showed higher P/AI at 1st service compared to GTAI (49.1% vs. 36.4%, P = 0.04), whilst, median days to pregnancy did not differ between the two groups. Overall, P/AI of primiparous cows tended to be better in comparison with multiparous cows (48.3% vs. 37.2%, P = 0.06). In conclusion, there was no significant difference regarding the efficacy of 5- and 7-day Ovsynch + PRID protocols. Moreover, a modified G7G protocol, with intermediate heat detection, resulted in overall better P/AI compared to TAI protocols and appears as a promising strategy to optimize estrus detection for 1st AI.
Related Concept Videos
Hormonal Control of the Ovarian Cycle
Before puberty, the hypothalamus releases GnRH in a low frequency, low amplitude pulsatile manner. This along with the immature hypothalamic-pituitary-gonadal axis activity, results in low estrogen levels and the absence of a fully functional ovarian cycle. At puberty, GnRH secretion increases in both frequency and...
Ovarian Cycle
Hormonal Regulation of the Menstrual Cycle
At puberty, GnRH begins a pulsatile release pattern, which triggers the anterior pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The frequency and amplitude of GnRH pulses vary across the menstrual cycle, with faster pulses favoring LH release and slower pulses favoring FSH...

