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Long Intergenic Noncoding RNA OIN1 Promotes Ovarian Cancer Growth by Modulating Apoptosis-Related Gene Expression

Toshihiko Takeiwa1,2, Yuichi Mitobe1, Kazuhiro Ikeda1

  • 1Division of Systems Medicine & Gene Therapy, Saitama Medical University, Hidaka, Saitama 350-1241, Japan.

International Journal of Molecular Sciences
|October 23, 2021
PubMed

Insights

A novel long intergenic noncoding RNA, OIN1, is overexpressed in ovarian cancer. Silencing OIN1 inhibits tumor growth and enhances apoptosis, identifying OIN1 as a potential therapeutic target for ovarian cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Advanced ovarian cancer has high mortality rates, necessitating novel therapeutic strategies.
  • Long intergenic noncoding RNAs (lincRNAs) are emerging as potential molecular targets due to their tissue-specific activities.

Purpose of the Study:

  • To investigate the role of a novel lincRNA, ovarian cancer long intergenic noncoding RNA 1 (OIN1), in ovarian cancer.
  • To evaluate OIN1 as a potential therapeutic target for ovarian cancer management.

Main Methods:

  • RNA sequencing was used to analyze OIN1 expression in ovarian cancer tissues and normal ovaries.
  • OIN1 was silenced using small interfering RNAs (siRNAs) in ovarian cancer cells.
  • Gene expression analysis was performed to assess the correlation between OIN1 and apoptosis-related genes (RASSF5, ADORA1).
  • In vivo tumor growth was evaluated in immunodeficient mice following OIN1-specific siRNA injection.

Main Results:

  • OIN1 was found to be overexpressed in ovarian cancer tissues compared to normal ovaries.
  • OIN1 silencing significantly inhibited ovarian cancer cell proliferation and induced apoptosis.
  • OIN1 expression was negatively correlated with apoptosis-related genes RASSF5 and ADORA1, which were upregulated upon OIN1 knockdown.
  • OIN1-specific siRNA injection suppressed in vivo tumor growth in a mouse model.

Conclusions:

  • OIN1 functions as a tumor-promoting lincRNA in ovarian cancer by modulating apoptosis.
  • OIN1 represents a promising molecular target for the development of new ovarian cancer therapies.
Keywords:
RNA sequencinglong intergenic noncoding RNA (lincRNA)ovarian cancersmall interfering RNA (siRNA)xenograft

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