Related Experiment Videos
Antimicrobial activity of imipenem against 1386 clinical isolates
Abstract:
1386 isolates from clinical specimens were tested against imipenem by disc agar diffusion. The bacteria used in this study consisted of Escherichia coli, Enterobacter aerogenes, E. agglomerans, E. cloacae, Klebsiella pneumoniae, K. oxytoca, K. ozanae, Proteus mirabilis, P. vulgaris, Providencia stuartii, P. rettgeri, Acinetobacter calcoaceticus, Citrobacter diversus, C. freundii, Morganella morganii, Serratia liquefaciens, S. marcescens, Hafnia alvei, Aeromonas hydrophila, Pseudomonas aeruginosa, P. cepacia, P. maltophila, P. fluorescens, Staphylococcus aureus, S. epidermidis, S. saprophyticus, pneumococcus, Lancefield group A, B and D streptococci, viridans streptococci, diphtheroids and Bacillus species. In vitro activity of imipenem was compared with the following antibiotics: ampicillin, amikacin, carbenicillin, cefoperazone, cefoxitin, cephalothin, chloramphenicol, clindamycin, colistin, erythromycin, gentamicin, methicillin, penicillin, tetracycline, tobramycin, trimethoprim-sulfamethoxazole and vancomycin. Of the 819 strains of Enterobacteriaceae tested, 99.5% were susceptible to imipenem. Ninety-seven percent strains of P. aeruginosa were also susceptible to imipenem. All the 161 isolates of S. aureus and 116 of the 117 isolates of enterococci exhibited in vitro susceptibility to this antibiotic. All gram positive bacteria tested were inhibited by imipenem except 28% isolates of S. epidermidis and 5% isolates of S. agalactiae.
Insights
Imipenem demonstrates high in vitro activity against a broad spectrum of clinical bacteria, including Enterobacteriaceae, Pseudomonas aeruginosa, Staphylococcus aureus, and enterococci. This broad-spectrum antibiotic is effective against most gram-positive bacteria tested.
Area of Science:
- Microbiology
- Infectious Diseases
- Pharmacology
Background:
- Antibiotic resistance is a growing global health concern, necessitating the evaluation of potent antimicrobial agents.
- Carbapenems, such as imipenem, are critical for treating severe bacterial infections.
Purpose of the Study:
- To assess the in vitro antimicrobial activity of imipenem against a diverse range of clinical bacterial isolates.
- To compare the efficacy of imipenem with other commonly used antibiotics.
Main Methods:
- Disc agar diffusion method was employed to test 1386 clinical bacterial isolates.
- Susceptibility testing included Gram-negative bacteria (Enterobacteriaceae, Pseudomonas aeruginosa) and Gram-positive bacteria (Staphylococcus aureus, enterococci, streptococci, etc.).
- Imipenem's activity was compared against a panel of 19 other antibiotics.
Main Results:
- High susceptibility rates were observed: 99.5% of Enterobacteriaceae, 97% of Pseudomonas aeruginosa, and nearly all Staphylococcus aureus and enterococci isolates were susceptible to imipenem.
- Imipenem effectively inhibited most Gram-positive bacteria, with exceptions including 28% of Staphylococcus epidermidis and 5% of Streptococcus agalactiae isolates.
Conclusions:
- Imipenem exhibits potent in vitro activity against a wide array of clinically significant bacteria.
- Its broad spectrum of activity makes it a valuable therapeutic option for serious infections caused by susceptible pathogens.