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Conjunctival Sac Microbiome in Infectious Conjunctivitis
Yasser Helmy Mohamed1, Masafumi Uematsu1, Yoshitomo Morinaga2
1Department of Ophthalmology and Visual Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan.
Microorganisms
|October 23, 2021
Summary
This study analyzed the bacterial communities in infectious conjunctivitis cases in Vietnam. Firmicutes, Actinobacteria, and Proteobacteria were the most abundant phyla, with potential implications for infection prevention.
Area of Science:
- Microbiology
- Ophthalmology
Background:
- Acute bacterial conjunctivitis is a prevalent ocular infection.
- Understanding the conjunctival microbiota is crucial for diagnosing and treating infections.
Purpose of the Study:
- To characterize the bacterial community composition in patients with infectious conjunctivitis.
- To identify key bacterial phyla and genera associated with bacterial conjunctivitis.
Main Methods:
- Conjunctival swabs were collected from 47 patients in Vietnam.
- Bacterial DNA was analyzed using conventional culture, PCR, and 16S ribosomal RNA sequencing.
- Diversity analysis was performed using PERMANOVA (unweighted UniFrac).
Main Results:
- Firmicutes, Actinobacteria, and Proteobacteria were the dominant bacterial phyla, comprising 92% of sequences.
- Streptococcus, Cutibacterium, and Staphylococcus were among the most abundant genera.
- Bilateral conjunctivitis and antibiotic use showed significant differences in bacterial community composition.
Conclusions:
- Firmicutes was the most abundant phyla in bacterial conjunctivitis cases.
- The resident commensal microbiota, such as Pseudomonas, may play a role in preventing infection.
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