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Updated: Oct 15, 2025

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Genome Analysis of Nicotinamide Adenine Dinucleotide-Independent Mycoplasma synoviae Isolates From Korea

Yongjun Song1, Tae-Min La1, Taesoo Kim1

  • 1College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.

Pathogens (Basel, Switzerland)
|October 23, 2021
PubMed

Insights

NAD-independent Mycoplasma synoviae (MS) strains were identified in Korea. Genomic analysis revealed unique genetic sequences in these avian pathogens, offering insights into their atypical characteristics.

Area of Science:

  • Avian pathology
  • Microbiology
  • Genomics

Background:

  • Mycoplasma synoviae (MS) is a significant avian pathogen causing respiratory and skeletal diseases in chickens.
  • NAD-independent MS strains, noted since 1975, possess atypical traits requiring further investigation.
  • The genetic basis for NAD independence in MS remains largely uncharacterized.

Purpose of the Study:

  • To investigate the genetic underpinnings of NAD independence in Mycoplasma synoviae.
  • To characterize novel NAD-independent MS strains isolated from Korea.

Main Methods:

  • Isolation and whole-genome sequencing of five NAD-independent MS strains from Korea.
  • Genome assembly using Illumina and Oxford Nanopore Technology platforms.
  • Comparative genomic analysis against reference MS strains (MS-H vaccine and WVU1853).

Main Results:

  • Successful assembly of genomes for five Korean NAD-independent MS isolates.
  • Identification of unique coding sequences absent in NAD-dependent strains.
  • Specific genes, including nicotinate phosphoribosyltransferase and glycerol-3-phosphate acyltransferase, were found to be present in NAD-independent isolates.

Conclusions:

  • The study identified unique genetic markers associated with NAD independence in Mycoplasma synoviae.
  • These findings provide a foundation for understanding the molecular mechanisms behind NAD-independent MS.
  • Further research can explore the functional significance of these unique sequences in avian disease pathogenesis.
Keywords:
Mycoplasma synoviaeNADchickencomparative analysisnicotinamide adenine dinucleotide

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