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Related Experiment Video

Updated: Oct 15, 2025

On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis
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Profiling of Carnitine Shuttle System Intermediates in Gliomas Using Solid-Phase Microextraction (SPME).

Joanna Bogusiewicz1, Katarzyna Burlikowska1, Karol Jaroch1

  • 1Department of Pharmacodynamics and Molecular Pharmacology, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 85-089 Bydgoszcz, Poland.

Molecules (Basel, Switzerland)
|October 23, 2021
PubMed
Summary

Carnitine and acylcarnitine levels in brain tumors may indicate cancer. Higher concentrations correlated with increased glioma malignancy and poorer clinical outcomes, suggesting potential for early detection and treatment strategies.

Keywords:
acylcarnitinecancercarnitinegliomaliquid chromatography–mass spectrometry LC–MSsolid-phase microextraction SPME

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Area of Science:

  • Biochemistry
  • Oncology
  • Analytical Chemistry

Background:

  • Alterations in the carnitine shuttle system are linked to cancer development.
  • Understanding carnitine profiles in tumors is crucial for cancer detection and treatment.

Purpose of the Study:

  • To analyze carnitine and acylcarnitine profiles in gliomas.
  • To correlate these profiles with glioma grade, IDH mutations, and 1p/19q co-deletion status.

Main Methods:

  • On-site solid-phase microextraction (SPME) of tumor samples immediately after excision.
  • Liquid chromatography-high-resolution mass spectrometry for analyte analysis.
  • Correlation analysis to identify patterns in carnitine and acylcarnitine levels.

Main Results:

  • SPME successfully extracted carnitine and 22 acylcarnitines.
  • Two distinct clusters of short-chain and long-chain carnitine esters were identified.
  • Elevated carnitine and acylcarnitine concentrations were observed in high-grade gliomas and samples with poorer clinical outcomes (IDH wild-type, 1p/19q non-co-deleted).

Conclusions:

  • The study demonstrates a novel chemical biopsy approach using SPME for on-site tumor sampling.
  • Carnitine and acylcarnitine profiles show potential as biomarkers for glioma malignancy and prognosis.
  • This method supports comprehensive multi-method analyses for improved cancer diagnostics.