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New Method for Simultaneous Arsenic and Selenium Speciation Analysis in Seafood and Onion Samples
Katarzyna Karaś1, Anetta Zioła-Frankowska2, Marcin Frankowski1
1Department of Analytical and Environmental Chemistry, Faculty of Chemistry, Adam Mickiewicz University in Poznan, 61-614 Poznan, Poland.
Molecules (Basel, Switzerland)
|October 23, 2021
Summary
This study introduces a rapid HPLC-ICP-MS method for simultaneous speciation analysis of arsenic and selenium in seafood and onions. The new technique efficiently separates various inorganic and organic forms of these elements within 10 minutes.
Area of Science:
- Analytical Chemistry
- Environmental Science
- Food Science
Background:
- Arsenic and selenium speciation is crucial for assessing toxicity and bioavailability.
- Existing methods for simultaneous speciation of multiple arsenic and selenium compounds can be time-consuming and complex.
- Seafood and onions are important dietary sources, necessitating accurate speciation analysis.
Purpose of the Study:
- To develop and validate a novel, rapid method for the simultaneous speciation analysis of arsenic and selenium.
- To apply the developed method to diverse food matrices, specifically seafood and onions.
- To achieve efficient separation of inorganic and organic arsenic and selenium species.
Main Methods:
- High-Performance Liquid Chromatography coupled with Inductively Coupled Plasma Mass Spectrometry (HPLC-ICP-MS).
- Utilized an anion exchange column (Dionex IonPac AS22) and a dual-layer guard column (Dionex Ion Pac CG5A) for species separation.
- Employed ammonium nitrate solution at pH 9.0 as the mobile phase.
Main Results:
- Successful simultaneous speciation of arsenic (arsenite, arsenate, DMA, MMA, AsB) and selenium (selenite, selenate, Se-Methionine, Se-Cystine).
- Achieved separation of all target species within a 10-minute run time with good resolution.
- Demonstrated applicability to complex matrices like seafood and onions.
Conclusions:
- The developed HPLC-ICP-MS method provides a fast and effective approach for simultaneous arsenic and selenium speciation.
- This method is suitable for routine analysis of these elements in various food samples.
- Accurate speciation analysis is vital for dietary exposure assessment and food safety.
