Related Experiment Video
Updated: Oct 15, 2025

Quantitative 31P NMR Analysis of Lignins and Tannins
Published on: August 2, 2021
Research Progress of NMR in Natural Product Quantification
Zhi-Fan Wang1, Yu-Lin You1, Fei-Fei Li1
1School of Pharmacy Sciences, Shandong University, 44# West Wenhua Road, Jinan 250012, China.
Abstract:
In the fields of medicine and health, traditional high-performance liquid chromatography or UV-visible spectrophotometry is generally used for substance quantification. However, over time, nuclear magnetic resonance spectroscopy (NMR) has gradually become more mature. Nuclear magnetic resonance spectroscopy has certain advantages in the quantitative analysis of substances, such as being nondestructive, having a high flux and short analysis time. Nuclear magnetic resonance spectroscopy has been included in the pharmacopoeiae of various countries. In this paper, the principle of nuclear magnetic resonance spectroscopy and the recent progress in the quantitative study of natural products by NMR are reviewed, and its application in the quantitative study of natural products is proposed. At the same time, the problems of using NMR alone to quantify natural products are summarized and corresponding suggestions are put forward.
Related Concept Videos
Applications Of NMR In Biology
¹H NMR Signal Integration: Overview
Two-Dimensional (2D) NMR: Overview
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse....
NMR Spectroscopy of Aromatic Compounds
Other Nuclides: 31P, 19F, 15N NMR
While fluorine-19 and phosphorous-31 have high natural abundances (100%) and positive gyromagnetic ratios, nitrogen-15 has a low natural abundance and a negative gyromagnetic ratio. However, nitrogen-15 is still preferred over nitrogen-14 (which has a...
2D NMR: Overview of Heteronuclear Correlation Techniques

