Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

267
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
267
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

6.5K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
6.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ferromagnetism versus Antiferromagnetism in Narrow-Band Systems: Competition between Quantum Geometry and Band Dispersion.

Physical review letters·2026
Same author

Variations of global brain asymmetry are associated with aging and related diseases.

Science advances·2026
Same author

<i>AaJAZ8</i> forms an extensive interaction network with <i>AaJAZ</i> proteins and two novel <i>AaMYC</i> transcription factors in <i>Artemisia annua</i>.

Frontiers in plant science·2026
Same author

Tandem duplication-driven expansion and UV-B stress adaptation of the LHC gene family in Artemisia annua L.

BMC plant biology·2026
Same author

Perivascular fat attenuation index on coronary computed tomography angiography predicts postoperative atrial fibrillation after off-pump coronary artery bypass grafting.

BMC cardiovascular disorders·2026
Same author

Construction of donkey skin fibroblast immortalized cell line and comparative analysis with primary cells.

Tissue & cell·2026

Related Experiment Video

Updated: Oct 15, 2025

Author Spotlight: Harnessing DNA Barcode Technology to Enhance the Efficiency of Medicinal Plant Identification
08:55

Author Spotlight: Harnessing DNA Barcode Technology to Enhance the Efficiency of Medicinal Plant Identification

Published on: November 1, 2024

1.9K

Licorice Germplasm Resources Identification Using DNA Barcodes Inner-Variants.

Qianwen Liu1, Shuai Guo2, Xiasheng Zheng3

  • 1Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.

Plants (Basel, Switzerland)
|October 23, 2021
PubMed
Summary

Accurate licorice seed authentication is vital for quality control. A new high-throughput full-length multiple DNA barcodes (HFMD) method using SMRT sequencing effectively determines licorice seed heterozygosity and composition.

Keywords:
DNA barcodinggermplasm resourceslicoricenext-generation sequencing (NGS)single-molecule real-time (SMRT) sequencing

More Related Videos

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
12:01

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

Published on: February 20, 2012

31.8K
Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA
13:16

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

Published on: January 22, 2018

21.7K

Related Experiment Videos

Last Updated: Oct 15, 2025

Author Spotlight: Harnessing DNA Barcode Technology to Enhance the Efficiency of Medicinal Plant Identification
08:55

Author Spotlight: Harnessing DNA Barcode Technology to Enhance the Efficiency of Medicinal Plant Identification

Published on: November 1, 2024

1.9K
A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
12:01

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

Published on: February 20, 2012

31.8K
Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA
13:16

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

Published on: January 22, 2018

21.7K

Area of Science:

  • Genetics
  • Pharmacognosy
  • Biotechnology

Background:

  • Accurate authentication of licorice seeds is crucial for quality control and clinical efficacy.
  • Challenges in licorice seed resource stability stem from their heterozygous, polyploid, and hybrid nature.

Purpose of the Study:

  • To develop a novel method for determining the heterozygosity and composition of licorice seed mixtures.
  • To evaluate the performance of high-throughput full-length multiple DNA barcodes (HFMD) for licorice seed analysis.

Main Methods:

  • Utilized high-throughput full-length multiple DNA barcodes (HFMD) targeting ITS, ITS2, and psbA-trnH regions.
  • Employed next-generation sequencing (NGS) and single-molecule real-time (SMRT) technologies for DNA sequencing.
  • Compared sequencing methods to identify the most effective approach for complex licorice seed samples.

Main Results:

  • Single-molecule real-time (SMRT) sequencing demonstrated superior performance in identifying complex mixed samples.
  • The HFMD method proved effective and feasible for assessing licorice seed heterozygosity.
  • The study successfully interpreted DNA barcoding for multi-base, multi-heterozygous, and polyploid species.

Conclusions:

  • HFMD combined with SMRT sequencing offers a robust solution for licorice seed authentication and quality control.
  • This approach can be applied to diverse and complex plant species with heterozygous and polyploid characteristics.
  • The findings contribute to ensuring the genetic stability and clinical efficacy of licorice resources.