Roe-inspired stem cell microcapsules for inflammatory bowel disease treatment
Guopu Chen1, Fengyuan Wang2, Min Nie1
1Department of Rheumatology and Immunology, Institute of Translational Medicine, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210002, China.
Summary
Roe-inspired microcapsules encapsulate mesenchymal stem cells (MSCs) for inflammatory bowel disease treatment. These nutrient-rich, immune-isolating capsules enhance MSC survival, function, and bowel adhesion, improving therapeutic outcomes.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Immunology
Background:
- Mesenchymal stem cells (MSCs) show promise for inflammatory bowel disease (IBD) but face challenges like immune attack and poor nutrient supply.
- Current MSC therapies for IBD are limited by low accumulation at target sites and immune system evasion.
- Developing advanced delivery systems is crucial to overcome these limitations for effective IBD treatment.
Purpose of the Study:
- To develop novel, immune-isolating, wet-adhesive, and nutrient-rich microcapsules for encapsulating therapeutic mesenchymal stem cells (MSCs).
- To mimic natural incubation mechanisms using roe-inspired materials for enhanced MSC survival and function.
- To evaluate the efficacy of these engineered MSC-loaded microcapsules in a preclinical model of inflammatory bowel disease.
Main Methods:
- Fabrication of adhesive microcapsule shells using alginate, epsilon-poly-L-lysine-graft-methacrylamide, and dopamine methacrylamide via ionic cross-linking and visible light curing.
- Encapsulation of MSCs and roe proteins within the microcapsule core to provide nutrients and immune protection.
- Intraperitoneal transplantation of microcapsules in a dextran sulfate sodium (DSS)-induced colitis model to assess therapeutic effects.
Main Results:
- The core-shell microcapsules successfully protected MSCs from immune attack while preserving their immunomodulatory functions.
- Encapsulated roe proteins provided essential nutrients, supporting MSC survival and proliferation.
- Wet-adhesive properties enabled microcapsule immobilization on the bowel, increasing residence time and demonstrating therapeutic benefits in DSS-induced colitis.
Conclusions:
- Roe-inspired microcapsules offer a promising strategy for encapsulating MSCs, overcoming key limitations for IBD therapy.
- The enhanced survival, immune protection, and targeted delivery of MSCs via these microcapsules suggest significant clinical potential.
- This biomimetic approach represents a novel advancement in stem cell-based therapies for inflammatory bowel disease.
Related Concept Videos
01:29Drugs for Treatment of Crohn's Disease in IBD Using Immunomodulatory Agents
286
Crohn's disease is an inflammatory bowel disorder marked by chronic inflammation of the GI tract. Various treatment strategies for Crohn's disease are employed, such as immunomodulatory agents, glucocorticoids, and biologics or anti-TNF therapy. Azathioprine (Imuran), a commonly used immunomodulatory drug for Crohn's disease, is converted in the body to mercaptopurine, which inhibits purine biosynthesis and cell proliferation. Both are utilized in severe cases of Inflammatory Bowel...
286
01:29Inflammatory Bowel Disease IV: Pharmacological Management
238
Upon diagnosis, managing Inflammatory Bowel Disease (IBD) involves addressing several crucial aspects. The primary goals include resting the bowel, correcting malnutrition, and providing symptomatic relief. Resting the bowel may consist of medications to reduce inflammation and promote healing. Correcting malnutrition is essential, often requiring dietary adjustments and nutritional supplements. Symptomatic relief aims to ease pain, diarrhea, and other discomforts in IBD.
Pharmacologic...
Pharmacologic...
238
01:21Drugs for Treatment of Crohn's Disease in IBD Using Glucocorticoids
238
Glucocorticoids, a class of anti-inflammatory drugs, are pivotal in treating moderate to severe Crohn's disease by inducing remission. They exhibit their anti-inflammatory action by inhibiting the production of inflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-1, and chemokines like IL-8. In addition, they reduce the expression of inflammatory cell adhesion molecules and inhibit gene transcription of nitric oxide synthase, phospholipase A2, cyclooxygenase-2...
238
01:23Renewal of Intestinal Stem Cells
2.8K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
2.8K
01:29Drugs for Treatment of Ulcerative Colitis in IBD
267
Ulcerative colitis is a chronic inflammatory condition primarily affecting the colon and rectum. The primary drugs used in the treatment of ulcerative colitis are aminosalicylates. They exhibit anti-inflammatory and immunosuppressive properties. They modulate inflammatory mediators and inhibit the activity of nuclear factor κB (NF-κB). Aminosalicylates also reduce inflammation by inhibiting prostaglandin and leukotriene production and decreasing neutrophil chemotaxis and superoxide...
267
01:30Inflammatory Bowel Disease II: Crohn's Disease
451
Introduction
Inflammatory bowel disease, commonly known as IBD, refers to a collection of disorders that lead to persistent inflammation of the gastrointestinal tract. The two types of IBD are ulcerative colitis, which impacts the colon, and Crohn's disease, which can involve any part of the gastrointestinal segment.
Crohn's disease
Crohn's disease is a chronic, systemic inflammatory bowel disease (IBD) that predominantly affects the gastrointestinal tract. It is marked by...
Inflammatory bowel disease, commonly known as IBD, refers to a collection of disorders that lead to persistent inflammation of the gastrointestinal tract. The two types of IBD are ulcerative colitis, which impacts the colon, and Crohn's disease, which can involve any part of the gastrointestinal segment.
Crohn's disease
Crohn's disease is a chronic, systemic inflammatory bowel disease (IBD) that predominantly affects the gastrointestinal tract. It is marked by...
451