Related Experiment Video

Updated: Oct 15, 2025

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

7.3K

Effect of tumor-associated macrophages on lncRNA PURPL/miR-363/PDZD2 axis in osteosarcoma cells

Fan He1, Guoming Ding2, Wu Jiang2

  • 1Department of Orthopedics, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, 261 HuanSha Road, ShangCheng, HangZhou, ZheJiang, 310006, China. FHe_Doc_29@yeah.net.

Cell Death Discovery
|October 23, 2021
PubMed

Insights

Tumor-associated macrophages promote osteosarcoma by upregulating lncRNA PURPL, which inhibits miR-363 and increases PDZD2 expression. This pathway drives tumor progression and migration.

Area of Science:

  • Oncology
  • Molecular Biology
  • Immunology

Background:

  • Tumor-associated macrophages (TAMs) are key players in osteosarcoma (OS) progression.
  • Previous studies indicated miR-363 inhibits OS cell growth by reducing PDZ domain containing 2 (PDZD2) expression.

Purpose of the Study:

  • To investigate the regulatory roles of TAMs on the miR-363/PDZD2 axis in osteosarcoma.
  • To elucidate the mechanism involving the long noncoding RNA p53 upregulated regulator of P53 levels (lncRNA PURPL) in TAM-mediated OS progression.

Main Methods:

  • TAM-like macrophages were generated from CD14+ peripheral blood mononuclear cells (PBMCs).
  • MG-63 cells were transfected with miR-363 mimics or inhibitors to assess TAM migration.
  • The regulatory effect of PURPL on miR-363 expression and its influence on MG-63 cell proliferation, migration, invasion, epithelial-mesenchymal transition (EMT), and TAM migration were analyzed.
  • PDZD2 knockdown was used to confirm PURPL's effects.

Main Results:

  • TAM-like macrophages were successfully induced.
  • miR-363 mimics suppressed TAM migration, while inhibitors promoted it.
  • TAMs increased PURPL expression in MG-63 cells, identifying PURPL as an upstream regulator of miR-363.
  • PURPL overexpression enhanced MG-63 cell proliferation, migration, invasion, EMT, and TAM migration, while knockdown had opposite effects.
  • PDZD2 silencing attenuated the effects of PURPL overexpression on MG-63 cells and TAM migration.

Conclusions:

  • TAMs promote osteosarcoma development by modulating the PURPL/miR-363/PDZD2 axis.
  • The lncRNA PURPL acts as a crucial mediator in TAM-driven osteosarcoma progression.
  • Targeting the PURPL/miR-363/PDZD2 pathway could offer therapeutic strategies for osteosarcoma.

More Related Videos

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports07:44

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Published on: November 28, 2019

7.7K
A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells11:15

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

Published on: May 6, 2018

10.3K

Related Concept Videos

The Tumor Microenvironment02:17

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
7.0K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.7K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.2K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.9K