Related Experiment Video

Updated: Oct 15, 2025

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
14:47

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Published on: May 17, 2016

10.0K

ZSWIM8 is a myogenic protein that partly prevents C2C12 differentiation

Fumihiko Okumura1, Nodoka Oki2, Yuha Fujiki2

  • 1Department of Food and Health Sciences, International College of Arts and Sciences, Fukuoka Women's University, Fukuoka, 813-8582, Japan. okumura@fwu.ac.jp.

Scientific Reports
|October 23, 2021
PubMed

Insights

Zinc finger SWIM type containing 8 (ZSWIM8) is a novel protein in the Cdon complex that regulates muscle cell differentiation. Knocking down ZSWIM8 accelerates myogenesis in C2C12 cells, indicating its inhibitory role.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Muscle Development

Background:

  • Cell adhesion molecule-related/downregulated by oncogenes (Cdon) is a cell-surface receptor crucial for myogenesis.
  • The Cdon complex, including JLP, Bnip-2, and CDC42, activates p38 MAPK to promote muscle differentiation.
  • The full protein composition of the Cdon complex during myogenesis remains incompletely understood.

Purpose of the Study:

  • To identify novel proteins within the Cdon complex during C2C12 cell differentiation.
  • To investigate the role of Cullin 2-interacting protein zinc finger SWIM type containing 8 (ZSWIM8) in myogenesis.
  • To determine if ZSWIM8 affects the stability of known Cdon complex components.

Main Methods:

  • C2C12 myoblast cell culture and differentiation induction.
  • Knockdown of ZSWIM8 using siRNA.
  • Western blotting to assess protein levels and ubiquitination.
  • Analysis of C2C12 cell differentiation markers.

Main Results:

  • ZSWIM8 expression is induced during C2C12 cell differentiation and ZSWIM8 is part of the Cdon complex.
  • ZSWIM8 knockdown accelerates C2C12 myogenesis.
  • Knockdown of ZSWIM8 did not alter the ubiquitination or stability of Bnip2, Cdon, or JLP.

Conclusions:

  • ZSWIM8 is a novel myogenic protein integrated into the Cdon complex.
  • ZSWIM8 functions as a negative regulator of C2C12 cell differentiation.
  • ZSWIM8 inhibits myogenesis independently of Bnip2, Cdon, or JLP protein stability.

More Related Videos

Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages07:51

Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages

Published on: April 30, 2014

20.6K
Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin shRNA12:19

Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin shRNA

Published on: February 12, 2020

8.0K

Related Concept Videos

Formation of Muscle Fibers from Myoblasts01:13

Formation of Muscle Fibers from Myoblasts

De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
5.3K
Satellite Stem Cells and Muscular Dystrophy01:21

Satellite Stem Cells and Muscular Dystrophy

Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
2.1K