Separating spin compartments in arterial spin labeling using delays alternating with nutation for tailored excitation...

To clarify the type of spin compartment in arterial spin labeling (ASL) that is eliminated by delays alternating with nutation for tailored excitation (DANTE) pulse using T₂ -relaxometry, and to demonstrate the feasibility of arterial cerebral blood volume (CBV_a ) imaging using DANTE-ASL in combination with a simplified two-compartment model.

The DANTE and T₂ -preparation modules were combined into a single ASL sequence. T₂ values under the application of DANTE were determined to evaluate changes in T₂ , along with the post-labeling delay (PLD) and the relationship between transit time without DANTE (TT_noVS ) and T₂ . The reference tissue T₂ (T_{2_ref} ) was also obtained. Subsequently, the DANTE module was embedded into the Hadamard-encoded ASL. Cerebral blood flow (CBF) and CBV_a were computed using two Hadamard-encoding datasets (with and without DANTE) in a rest and breath-holding (BH) task.

While T₂ without DANTE (T_{2_noVS} ) decreased as the PLD increased, T₂ with DANTE (T_{2_DANTE} ) was equivalent to T_{2_ref} and did not change with the PLD. Although there was a significant positive correlation between TT_noVS and T_{2_noVS} with short PLD, T_{2_DANTE} was not correlated with TT_noVS nor PLD. Baseline CBV_a values obtained at rest were 0.64 ± 0.12, 0.64 ± 0.11, and 0.58 ± 0.15 mL/100 g for anterior, middle, and posterior cerebral arteries, respectively. Significant CBF and CBV_a elevations were observed in the BH task.

Microvascular compartment signals were eliminated from the total ASL signals by DANTE. CBV_a can be measured using Hadamard-encoded DANTE-ASL in combination with a simplified two-compartment model.