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Updated: Oct 15, 2025

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
Published on: May 10, 2024
Using a multi-head, convolutional neural network with data augmentation to improve electropherogram classification
1Forensic Science South Australia, 21 Divett Place, Adelaide, SA 5000, Australia; Flinders University, GPO Box 2100, Adelaide, SA 5001, Australia.
Abstract:
DNA profiles are generated in forensic biology laboratories around the world. It is possible that these profiles are assessed by two independent people in order for the profiles to be 'read'. Recent work has been carried out to develop a neural network model to classify fluorescence in a DNA profile electropherogram and potentially replace one, or both human readers. The ability to use neural networks for this function has been programmed into the software FaSTR™ DNA, which has been validated for use in at least one laboratory in Australia. The work that previously developed a neural network system had a number of limitations, specifically it was computer intensive, did not make the best use of available data, and consequently the performance of this model was sub-optimal in some conditions (particularly for low-intensity peaks). In the current work a new neural network model is developed that makes various improvements on the old model, by using convolutional layers, a multi-head architecture and data augmentation. Results indicate that an improved performance can be expected for low-intensity profiles.

