Hybrid catalyst cascade for enhanced oxidation of glucose in glucose/air biofuel cell

Redox enzymes are capable of harvesting electrical energy from biofuels in high catalytic activity and under mild condition. However, it is difficult to achieve efficient electron transfer and deep oxidation of biofuels simultaneously in a single-enzyme catalytic system. Herein, we report a hybrid catalyst cascade consisting of an organic oxidation catalyst, 2,2,6,6-tetramethyl-1-piperidine N-oxyl (TEMPO), and an enzyme, glucose oxidase (GOx), for electrochemical oxidation of glucose. It is found that TEMPO is capable of mediating electron transfer between the redox center of GOx and the electrode surface. While glucose can be oxidized into glucuronic acid under neutral conditions. Thus, combining GOx and TEMPO, we are able to achieve 4e^- electrooxidation of glucose using the hybrid enzymatic and organic cascade (HEOC) system. When coupled with an air-breathing Pt cathode, the resulting glucose/air biofuel cell using the proposed HEOC anode exhibits a maximum power density of 38.1 μW cm^-2 with a short-circuit current of 651.4 μA cm^-2, which can be attributed to the enhanced energetic efficiency, enabling TEMPO a promising catalyst for glucose oxidation in bioelectronics applications.