Related Experiment Video

Updated: Oct 15, 2025

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin
10:51

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin

Published on: July 14, 2017

31.9K

Down-Regulated Calcium-Sensing Receptor in Keratinocytes and Skin from Aged Mice and Humans Impairs Function

Wendy B Bollag1

  • 1Charlie Norwood VA Medical Center, Augusta, Georgia, USA; Department of Physiology, Medical College of Georgia, Augusta University, Augusta, Georgia, USA; Department of Dermatology, Medical College of Georgia, Augusta University, Augusta, Georgia, USA; Department of Medicine, Medical College of Georgia, Augusta University, Augusta, Georgia, USA.

The Journal of Investigative Dermatology
|October 24, 2021
PubMed

Insights

The calcium-sensing receptor (CaSR) is crucial for skin health and wound repair. Reduced CaSR in aged skin impairs healing, but CaSR agonists show promise as a therapy for age-related skin repair delays.

Area of Science:

  • Dermatology
  • Cell Biology
  • Aging Research

Background:

  • The calcium-sensing receptor (CaSR) plays a vital role in skin physiology.
  • Key epidermal functions supported by CaSR include differentiation, barrier repair, and wound healing.

Purpose of the Study:

  • To investigate the role of CaSR in skin aging and wound healing.
  • To explore the therapeutic potential of CaSR agonists for age-related skin defects.

Main Methods:

  • Analysis of CaSR levels in aged skin and keratinocytes.
  • Assessment of epidermal functions in aged skin models.
  • Evaluation of CaSR agonist efficacy in correcting age-related functional impairments.

Main Results:

  • CaSR levels are significantly reduced in keratinocytes and skin from aged individuals.
  • Reduced CaSR is associated with impaired epidermal differentiation, barrier repair, and wound healing.
  • CaSR agonists effectively restored these impaired functions in aged skin models.

Conclusions:

  • Decreased CaSR expression contributes to the functional decline of aged skin.
  • CaSR agonists represent a potential therapeutic strategy to enhance skin repair and combat aging-related wound healing delays.

More Related Videos

Generation and Culturing of Primary Human Keratinocytes from Adult Skin10:42

Generation and Culturing of Primary Human Keratinocytes from Adult Skin

Published on: December 22, 2017

16.7K
Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair07:32

Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair

Published on: February 17, 2021

7.7K

Related Concept Videos

The Effect of Aging on Tissues01:19

The Effect of Aging on Tissues

Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...
2.9K
Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
2.7K
Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.4K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.3K
Bone Disorders01:29

Bone Disorders

Aging and its effect on bone remodeling is the most common cause of bone disorders. In young and healthy people, bone deposition and resorption happen at an equal rate to maintain optimal bone health.
Bone deposition is also affected by the levels of sex hormones like estrogen and testosterone that promote osteoblast activity and bone matrix synthesis. When the level of these hormones decreases due to aging, it causes a reduction in bone deposition. As a result, bone resorption by osteoclasts...
4.2K