CircRNA NRIP1 promotes papillary thyroid carcinoma progression by sponging mir-195-5p and modulating the P38 MAPK and

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In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
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In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

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Chuang Li1,2, Lijuan Zhu3, Lijun Fu1

  • 1Department of Thyroid Surgery, The First Affiliated Hospital of Zhengzhou University, Νo. 1 Jianshe East Road, 450052, Zhengzhou, China.

Diagnostic Pathology
|October 25, 2021
PubMed
Abstract

Insights

Circular RNA NRIP1 promotes papillary thyroid carcinoma (PTC) by increasing cell proliferation and invasion while inhibiting apoptosis. It sponges miR-195-5p, affecting P38 MAPK and JAK/STAT pathways in PTC progression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Circular RNAs (circRNAs) are crucial in tumor biology due to their regulatory roles.
  • Papillary thyroid carcinoma (PTC) is a significant area of oncological research.
  • The specific function of circRNA nuclear receptor-interacting protein 1 (circNRIP1) in PTC remains unclear.

Purpose of the Study:

  • To investigate the role of circRNA NRIP1 in papillary thyroid carcinoma (PTC).
  • To elucidate the underlying molecular mechanisms of circNRIP1 in PTC progression.
  • To explore the relationship between circNRIP1, miR-195-5p, and signaling pathways in PTC.

Main Methods:

  • Quantitative real-time PCR to assess circNRIP1 expression in PTC tissues and cell lines.
  • In vitro assays (MTT, Transwell, flow cytometry) to evaluate the functional impact of circNRIP1 and miR-195-5p on PTC cells.
  • Dual-luciferase reporter and pull-down assays to confirm the interaction between circNRIP1 and miR-195-5p.
  • Murine xenograft models to study the in vivo effects of circNRIP1 and miR-195-5p.
  • Western blot analysis to examine the modulation of P38 MAPK and JAK/STAT signaling pathways.

Main Results:

  • CircRNA NRIP1 is significantly overexpressed in PTC tissues and cells, correlating with advanced tumor stage.
  • CircRNA NRIP1 depletion suppresses PTC cell proliferation and invasion while promoting apoptosis.
  • Overexpression of miR-195-5p inhibits PTC cell growth and tumor xenograft development, whereas circNRIP1 overexpression reverses these effects.
  • CircRNA NRIP1 upregulates p-p38, p-JAK2, and p-STAT1 protein levels, while miR-195-5p downregulates them.

Conclusions:

  • CircRNA NRIP1 acts as a tumor promoter in PTC by enhancing cell proliferation, invasion, and tumor growth.
  • CircRNA NRIP1 facilitates PTC progression by sponging miR-195-5p.
  • The mechanism involves the regulation of P38 MAPK and JAK/STAT signaling pathways by the circNRIP1/miR-195-5p axis.
Keywords:
CircRNA NRIP1JAK/STAT pathwayP38 MAPK pathwayPapillary thyroid carcinomamiR-195-5p

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