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Related Experiment Video

Updated: Oct 15, 2025

Building Finite Element Models to Investigate Zebrafish Jaw Biomechanics
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Cells at the Edge: The Dentin-Bone Interface in Zebrafish Teeth.

Joana T Rosa1,2, Paul Eckhard Witten1, Ann Huysseune1

  • 1Research Group Evolutionary Developmental Biology, Biology Department, Ghent University, Ghent, Belgium.

Frontiers in Physiology
|October 25, 2021
PubMed
Summary

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Standardization of bone morphometry and mineral density assessments in zebrafish and other small laboratory fishes using X-ray radiography and micro-computed tomography.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Researchยท2024

Zebrafish "bone of attachment" cells are osteoblasts, not odontoblasts, secreting a dentin-like tissue. This finding clarifies tooth evolution and mineralized tissue development in fish.

Area of Science:

  • Developmental Biology
  • Evolutionary Biology
  • Comparative Anatomy

Background:

  • Osteoblasts and odontoblasts, crucial for bone and dentin formation, share evolutionary origins.
  • Teleost fish exhibit diverse tooth attachment modes, with a debated
  • bone of attachment
  • tissue mediating dentin-jaw bone connections.

Purpose of the Study:

  • To characterize the cellular and tissue nature of the zebrafish
  • bone of attachment
  • using gene expression analysis.
  • To investigate the evolutionary relationship between bone and dentin formation in teleost fish.

Main Methods:

  • Utilized scpp5 (secretory calcium-binding phosphoprotein 5) expression to identify cells in the zebrafish
Keywords:
bonedentindermal skeletonodontoblastosteoblastscpptooth attachmentzebrafish

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  • bone of attachment
  • and surrounding tissues.
  • Employed Zns-5, an osteoblast marker, to further differentiate cell types.
  • Histological analysis to examine tissue characteristics, including cell extensions and osteocyte presence.

    • Main Results:

    • scpp5 was not expressed in cells forming the
    • bone of attachment
    • or in jaw bone osteoblasts, but was found in odontoblasts.
    • Cells of the
    • bone of attachment
    • expressed the osteoblast marker Zns-5.
    • The
    • bone of attachment
    • exhibited dentinal tubule-like extensions and lacked osteocytes, suggesting an intermediate tissue type, termed
    • dentinous bone
    • .

    Conclusions:

    • The cells producing the zebrafish
    • bone of attachment
    • are osteoblasts, and the tissue is a hybrid, best described as
    • dentinous bone
    • .
    • This research provides insights into the continuum of mineralized tissues and the divergence of osteoblast and odontoblast differentiation pathways.
    • The findings aid in understanding the evolutionary history of tooth attachment structures in actinopterygians.