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Diffusion of ofloxacin into the cerebrospinal fluid in patients with bacterial meningitis
Abstract:
Ofloxacin diffusion into cerebrospinal fluid (CSF) was evaluated in nine patients with bacterial meningitis. Patients were under treatment with i.v. amoxicillin (100 mg/kg/day), and during the first five days they were also given oral ofloxacin, 200 mg b.i.d. On days 2 and 5, blood and CSF samples were collected for assays by both HPLC and microbiological techniques. Samples were obtained 2 h (n = 2), 4 h (n = 1), 6 h (n = 2), 8 h (n = 2) and 12 h (n = 2) after the ofloxacin dose respectively. Concentration in CSF was 50 to 60% of that in serum and there was no significant difference between results with the two assay techniques.
Insights
This study shows oral ofloxacin effectively penetrates cerebrospinal fluid (CSF) in bacterial meningitis patients, reaching 50-60% of serum concentrations. This finding supports ofloxacin
Area of Science:
- Pharmacokinetics
- Infectious Diseases
- Neuroscience
Background:
- Bacterial meningitis requires effective antibiotic penetration into the central nervous system.
- Ofloxacin is a fluoroquinolone antibiotic with potential utility in treating CNS infections.
Purpose of the Study:
- To evaluate the diffusion of ofloxacin into the cerebrospinal fluid (CSF) of patients with bacterial meningitis.
- To compare CSF and serum concentrations of ofloxacin.
Main Methods:
- Nine patients with bacterial meningitis received oral ofloxacin (200 mg b.i.d.) concurrently with intravenous amoxicillin.
- Blood and CSF samples were collected at various time points (2, 4, 6, 8, 12 hours) post-dose on days 2 and 5.
- Ofloxacin concentrations were determined using High-Performance Liquid Chromatography (HPLC) and microbiological assays.
Main Results:
- Ofloxacin concentrations in CSF ranged from 50% to 60% of concurrent serum concentrations.
- Both HPLC and microbiological techniques yielded comparable results, indicating assay reliability.
- The study demonstrated consistent ofloxacin penetration into the CSF.
Conclusions:
- Oral ofloxacin achieves significant concentrations in the CSF of patients with bacterial meningitis.
- The findings support the use of ofloxacin as a therapeutic option for bacterial meningitis.
- Ofloxacin's penetration profile suggests its potential efficacy in treating CNS infections.