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Influence of ofloxacin on murine Peyer's patch lymphocytes
Abstract:
Several factors must be considered before the clinical impact of antimicrobials on the immune function can be determined. No data are available as yet on the influence of ofloxacin, a new fluoro-quinolone, on the lymphocytes of Peyer's patches. The present study offers evidence that murine Peyer's patches are influenced by ofloxacin administered by the oral route for seven days (15 mg/kg). No modifications of the percentage of the Thy 1,2 and SIg positive splenocytes and of the PHA and LPS-induced proliferative responses were observed studying cell suspensions from the spleen of the same animals. An immunotoxic effect of the compound on the cells of Peyer's patches or an impairment of their homing tendency has been postulated.
Insights
Ofloxacin, a fluoroquinolone antibiotic, may impact immune function by affecting Peyer's patch lymphocytes in mice. Spleen immune cell responses remained unchanged, suggesting a localized effect on gut immunity.
Area of Science:
- Immunology
- Pharmacology
- Microbiology
Background:
- Assessing antimicrobial effects on immune function requires careful consideration of various factors.
- Limited data exists on the impact of ofloxacin, a novel fluoroquinolone, on Peyer's patch lymphocytes.
Purpose of the Study:
- To investigate the influence of oral ofloxacin on murine Peyer's patch lymphocytes.
- To evaluate the effects of ofloxacin on spleen immune cell populations and responses.
Main Methods:
- Murine model administered oral ofloxacin (15 mg/kg) for seven days.
- Analysis of Peyer's patch lymphocyte populations.
- Flow cytometry assessment of Thy 1,2 and SIg positive splenocytes.
- Evaluation of PHA and LPS-induced splenocyte proliferative responses.
Main Results:
- Oral ofloxacin administration influenced murine Peyer's patches.
- No significant modifications were observed in the percentage of Thy 1,2 and SIg positive splenocytes.
- No alterations in PHA and LPS-induced proliferative responses of spleen cell suspensions were detected.
Conclusions:
- Ofloxacin may exert an immunotoxic effect specifically on Peyer's patch lymphocytes.
- Alternatively, ofloxacin might impair the homing tendency of these immune cells.
- Further research is needed to elucidate the precise mechanism of ofloxacin's impact on gut-associated lymphoid tissue.