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Updated: Oct 15, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
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Gene editing in plants: progress and challenges.

Yanfei Mao1, Jose Ramon Botella2, Yaoguang Liu3

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|October 25, 2021
PubMed
Summary
This summary is machine-generated.

The CRISPR-Cas9 genome editing tool revolutionizes plant science and crop breeding with its efficiency. Future plant genome editing research must address efficient delivery and precise gene editing strategies.

Keywords:
CRISPRCas9base editingcrop breedinggene targetinggenome editing

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Area of Science:

  • Agricultural Science
  • Molecular Biology
  • Genetics

Background:

  • The Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated protein 9 (Cas9) system offers precise gene editing capabilities.
  • CRISPR/Cas9 technology has significantly advanced plant genetic studies and crop improvement due to its efficiency and flexibility.

Purpose of the Study:

  • To provide an expert viewpoint on the current state and future trajectory of plant genome editing.
  • To highlight key challenges and potential concerns in the development and application of CRISPR/Cas9 in plants.

Main Methods:

  • Review and synthesis of current research on CRISPR/Cas9 applications in plants.
  • Analysis of existing methodologies for gene editing in diverse plant species.

Main Results:

  • Significant progress has been achieved in optimizing CRISPR/Cas9 for targeted gene mutagenesis in plants.
  • Current limitations include efficient delivery of editing tools and effective strategies for gene knock-ins and replacements.

Conclusions:

  • Plant genome editing holds immense potential for crop breeding and agricultural innovation.
  • Addressing challenges in delivery and precise editing is crucial for the widespread adoption and success of these technologies.