Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

766
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
766
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

591
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
591
Molecular Shapes01:18

Molecular Shapes

59.3K
Molecules have characteristic shapes that are crucial for their function. The arrangement of various electron groups around the central atom dictates their molecular geometry. Electron pairs in the valence shell of a central atom will adopt an arrangement that minimizes repulsions between the electron pairs by maximizing the distance between them. The valence electrons form either bonding pairs, located primarily between bonded atoms, or lone pairs.
Two regions of electron density in a diatomic...
59.3K
Molecular Models02:00

Molecular Models

41.7K
Physical models representing molecular architectures of chemical compounds play essential roles in understanding chemistry. The use of molecular models makes it easier to visualize the structures and shapes of atoms and molecules.
41.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-Redox-Potential p-Type Covalent Organic Frameworks With Abundant Active Sites for High-Voltage and Long-Life Lithium-Ion Battery Cathodes.

Angewandte Chemie (International ed. in English)·2026
Same author

Functional Microbiomes at the Interface: Mediators in Marine Biofouling and Larval Settlement.

International journal of molecular sciences·2026
Same author

A novel full-thickness skin graft technique for the surgical management of congenital buried penis in children.

Asian journal of andrology·2026
Same author

Explainable machine learning model for predicting traumatic brain injury-induced coagulopathy in elderly patients: A multicenter cohort study.

Neurosurgical review·2026
Same author

Bacterial-derived γ-aminobutyric acid (GABA) regulates larval settlement and metamorphosis of <i>Mytilus coruscus</i>.

Biofouling·2026
Same author

Fast Multi-view Discrete Clustering via Spectral Embedding Fusion.

IEEE transactions on pattern analysis and machine intelligence·2025

Related Experiment Video

Updated: Oct 15, 2025

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

4.1K

Visually constructing the chemical structure of a single molecule by scanning Raman picoscopy.

Yao Zhang1, Ben Yang1, Atif Ghafoor1

  • 1Hefei National Laboratory for Physical Sciences at the Microscale and Synergetic Innovation Center of Quantum Information and Quantum Physics, University of Science and Technology of China, Hefei 230026, China.

National Science Review
|October 25, 2021
PubMed
Summary

Researchers visualized single molecule vibrations using scanning Raman picoscopy. This technique allows ångström-level imaging, enabling molecular structure determination through a novel Lego-like assembly process.

Keywords:
interference effectscanning Raman picoscopystructure determinationtip-enhanced Raman spectroscopyvibrational mode imaging

More Related Videos

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
09:13

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

7.7K
Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures
08:49

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Published on: December 1, 2023

1.6K

Related Experiment Videos

Last Updated: Oct 15, 2025

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

4.1K
Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
09:13

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

7.7K
Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures
08:49

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Published on: December 1, 2023

1.6K

Area of Science:

  • Molecular spectroscopy
  • Nanophotonics
  • Chemical imaging

Background:

  • Nanocavity plasmonic fields offer strong spatial confinement for high-resolution molecular imaging.
  • Previous advancements allowed visualization of single molecule structures and vibrational modes.
  • Achieving full vibrational imaging is key to revealing detailed molecular structures.

Purpose of the Study:

  • To demonstrate ångström-level Raman imaging of individual vibrational modes in a single molecule.
  • To introduce a new methodology, scanning Raman picoscopy, for molecular structure determination.
  • To showcase the potential of ultrahigh-resolution spectromicroscopic images for visual molecular assembly.

Main Methods:

  • Utilizing strong spatial confinement of nanocavity plasmonic fields.
  • Performing full Raman imaging at the ångström level for a single Mg-porphine molecule.
  • Exploiting interference effects and Raman fingerprint databases for structural analysis.

Main Results:

  • Achieved ångström-level resolution for full Raman images of individual vibrational modes in a single Mg-porphine molecule.
  • Revealed distinct spatial characteristics for each vibrational mode.
  • Demonstrated the feasibility of scanning Raman picoscopy for molecular structure determination.

Conclusions:

  • Scanning Raman picoscopy provides unprecedented detail on molecular vibrations.
  • This technique enables visual, Lego-like assembly of molecular structures.
  • Ångström-level vibrational imaging opens new avenues for molecular structure elucidation.