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Related Experiment Videos

A cell surface receptor complex for collagen type I recognizes the Arg-Gly-Asp sequence.

S Dedhar, E Ruoslahti, M D Pierschbacher

    The Journal of Cell Biology
    |March 1, 1987
    PubMed
    Summary

    Researchers identified three cell surface proteins that act as receptors for type I collagen. These receptors bind collagen via the Arg-Gly-Asp sequence, suggesting a specific conformational recognition mechanism.

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    Area of Science:

    • Cell Biology
    • Biochemistry
    • Molecular Medicine

    Background:

    • Cell surface proteins mediate interactions between cells and their extracellular matrix.
    • Type I collagen is a major component of bone and connective tissues, crucial for cell adhesion and signaling.
    • The Arg-Gly-Asp (RGD) sequence is a known cell attachment motif found in various extracellular matrix proteins.

    Purpose of the Study:

    • To identify and characterize cell surface proteins that bind to type I collagen.
    • To investigate the role of the Arg-Gly-Asp (RGD) sequence in collagen-receptor interactions.
    • To elucidate the mechanism of type I collagen recognition by cell surface receptors.

    Main Methods:

    • Affinity chromatography using type I collagen and a collagen-like peptide matrices.

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  • Surface iodination of MG-63 human osteosarcoma cells.
  • Elution with Arg-Gly-Asp (RGD) containing peptides.
  • Liposome binding assays with purified polypeptides.

    • Main Results:

    • Three radioactive polypeptides (250 kD, 70 kD, 30 kD) showed specific affinity for collagen and the collagen-like peptide.
    • These polypeptides were specifically eluted by an RGD-containing peptide (Gly-Arg-Gly-Asp-Thr-Pro).
    • Liposomes incorporating these polypeptides bound specifically to type I collagen and the RGD peptide, but not to denatured collagen, fibronectin, or vitronectin.

    Conclusions:

    • The three identified polypeptides form a cell surface receptor complex for type I collagen.
    • The receptor interaction with type I collagen is mediated by the Arg-Gly-Asp (RGD) tripeptide sequence.
    • The conformation of the RGD sequence within collagen is critical for recognition by this receptor complex.