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Method for controlled tissue theranostics using a single tunable laser source.

Rok Podlipec1,2, Jaka Mur3, Jaka Petelin3

  • 1Laboratory of Biophysics, Condensed Matter Physics Department, Jožef Stefan Institute, Jamova cesta 39, Ljubljana, Slovenia.

Biomedical Optics Express
|October 25, 2021
PubMed
Summary
This summary is machine-generated.

This study introduces a novel real-time controlled tissue theranostics method for retinal diseases. It uses a tunable laser and advanced imaging to precisely monitor and guide laser treatments, improving therapeutic outcomes.

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Area of Science:

  • Biomedical Engineering
  • Ophthalmology
  • Laser Medicine

Background:

  • Theranostics combines diagnostics and therapeutics for tissue diseases.
  • Retinal therapies face challenges in treatment success, local metabolism improvement, and immediate feedback on laser effects.
  • Current methods lack real-time control and precise monitoring of laser-tissue interactions.

Purpose of the Study:

  • To present a proof-of-concept for real-time controlled tissue theranostics using a single tunable picosecond laser.
  • To demonstrate the capability of monitoring and quantifying laser-induced tissue changes in ex-vivo human retinal pigment epithelium.
  • To develop an optimized theranostics algorithm for personalized medicine applications.

Main Methods:

  • Utilized a single tunable picosecond (ps) laser source with adjustable irradiance, fluence, and repetition rate.
  • Employed ex-vivo human retinal pigment epithelium for proof-of-concept studies.
  • Applied autofluorescence intensity and lifetime imaging for diagnostics.
  • Developed a novel algorithm for optimized theranostics based on fluorescence parameters.

Main Results:

  • Autofluorescence intensity and lifetime imaging effectively recognized and quantified laser effects, from non-destructive modification to ablation.
  • The developed algorithm accurately quantified laser-induced tissue changes using fluorescence lifetime and intensity.
  • Demonstrated real-time control over laser-tissue interactions for precise therapeutic interventions.

Conclusions:

  • The developed real-time controlled tissue theranostics approach shows promise for retinal diseases and beyond.
  • This method offers a new paradigm for personalized medicine by enabling adaptable laser treatments with immediate feedback.
  • Autofluorescence diagnostics combined with an optimized algorithm provide a powerful tool for precise tissue modification and treatment monitoring.