Related Experiment Video

Updated: Oct 15, 2025

Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane
05:36

Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane

Published on: February 8, 2020

9.1K

A Novel ZNF304/miR-183-5p/FOXO4 Pathway Regulates Cell Proliferation in Clear Cell Renal Carcinoma

Li-Xin Ren1, Bo-Wen Zeng2, Meng Zhu1

  • 1Department of Urology, The Second Hospital of Hebei Medical University, Shijiazhuang, China.

Frontiers in Oncology
|October 25, 2021
PubMed

Insights

Zinc-finger protein 304 (ZNF304) is downregulated in clear cell renal carcinoma (ccRCC), promoting tumor growth. Targeting the ZNF304/miR-183-5p/FOXO4 axis may offer new ccRCC therapies.

Area of Science:

  • Molecular Biology
  • Oncology
  • Gene Regulation

Background:

  • Zinc-finger protein 304 (ZNF304) is crucial for gene silencing and cellular processes.
  • The role and clinical significance of ZNF304 in clear cell renal carcinoma (ccRCC) are not well understood.

Purpose of the Study:

  • To investigate the role of ZNF304 in ccRCC progression.
  • To elucidate the molecular mechanism underlying ZNF304's function in ccRCC.

Main Methods:

  • Analysis of ZNF304 expression in ccRCC tissues.
  • In vitro studies on ccRCC cell growth.
  • Investigation of the miR-183-5p/FOXO4 pathway.
  • ZNF304 binding to the miR-183 promoter was assessed.

Main Results:

  • ZNF304 expression is downregulated in ccRCC and correlates with poor survival.
  • Reduced ZNF304 promotes ccRCC cell growth; its overexpression inhibits growth.
  • The ZNF304/miR-183-5p/FOXO4 axis regulates ccRCC cell growth, with ZNF304 inhibiting miR-183-5p transcription and promoting FOXO4 expression.
  • miR-183-5p is upregulated in ccRCC, associated with poor prognosis, and promotes ccRCC progression by repressing FOXO4.

Conclusions:

  • The ZNF304/miR-183-5p/FOXO4 axis is a key driver of ccRCC progression.
  • This axis represents a potential therapeutic target for ccRCC treatment.
Keywords:
FOXO4clear cell renal carcinoma (ccRCC)miR-183-5pproliferationzinc-finger protein 304

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