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Novel lncRNA-miRNA-mRNA Competing Endogenous RNA Triple Networks Associated Programmed Cell Death in Heart Failure

Yu Zheng1, Yingjie Zhang1, Xiu Zhang1

  • 1Department of Rehabilitation Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Frontiers in Cardiovascular Medicine
|October 25, 2021
PubMed

Insights

This study reveals novel competing endogenous RNA (ceRNA) networks involving long non-coding RNA GAS5 in heart failure (HF). These networks regulate programmed cell death, offering new insights into HF pathophysiology.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cardiovascular Diseases

Background:

  • Long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) form regulatory networks crucial in cardiovascular diseases.
  • The specific role of lncRNA-mediated competing endogenous RNA (ceRNA) networks in heart failure (HF) pathogenesis remains largely unexplored.

Purpose of the Study:

  • To investigate the function of lncRNA-mediated ceRNA networks in the pathophysiological processes of HF.
  • To explore the regulatory roles of these networks in programmed cell death during HF development.

Main Methods:

  • Bioinformatic analysis of public datasets (GSE77399, GSE52601, GSE57338) to identify differentially expressed lncRNAs, miRNAs, and mRNAs.
  • Construction of lncRNA-miRNA-mRNA ceRNA networks and enrichment analysis (GO, KEGG) to predict biological functions.
  • Validation of identified ceRNA regulatory pathways related to programmed cell death using qRT-PCR.

Main Results:

  • Two lncRNA-mediated ceRNA networks, comprising two lncRNAs, eight miRNAs, and 65 mRNAs, were constructed for HF.
  • Enrichment analysis indicated involvement in extracellular matrix biological processes.
  • Seven specific lncRNA GAS5-mediated ceRNA pathways impacting programmed cell death (apoptosis, ferroptosis, pyroptosis) were identified and validated.

Conclusions:

  • Novel ceRNA regulatory networks involving lncRNA GAS5 in HF were discovered through bioinformatic analysis.
  • These networks are hypothesized to play significant roles in programmed cell death pathways relevant to HF.
  • The findings provide new perspectives and potential research directions for understanding ceRNA networks in HF.

Keywords:
apoptosiscompetitive endogenous RNAferroptosisheart failurelong non-coding RNAprogrammed cell death

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