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Updated: Oct 15, 2025

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
Published on: July 28, 2017
Characterization of the complete chloroplast genome of Actinidia hemsleyana
Qi Xiaoqiong1,2, Xie Xiaodong1,2, Yang Mingjuan3
1Hubei Key Laboratory of Purification and Application of Plant Anti-cancer Active Ingredients, School of Chemistry and Life Science, Hubei University of Education.
Abstract:
The completed chloroplast genome sequence of Actinidia hemsleyana, collected from eastern China, was firstly determined using next-generation sequencing (NGS) and bioinformatic analysis. The length of cp genome was 156,845 bp, including a large single-copy (LSC) region of 88,666 bp and a small single-copy (SSC) region of 20,543 bp, which were separated by a pair of inverted repeats (IRs) of 23,818 bp. The cp genome contained 132 genes, including 83 protein-coding genes, 41 tRNA genes, and 8 rRNA genes. The overall GC content was 37.19%, whereas the corresponding values of the LSC, SSC, and IR regions were 35.46%, 31.09%, and 43.06%, respectively. Phylogenetic analysis based on whole cp genome sequences of 20 Actinidia species showed that A. hemsleyana has the closest relationship with A.latifolia.
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