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Updated: Oct 15, 2025
A Universal Protocol for Large-scale gRNA Library Production from any DNA Source
Published on: December 6, 2017
Bioorthogonal Self-Immolative Linker Based on Grob Fragmentation
Xhenti Ferhati1, Marina Salas-Cubero1, Pablo Garrido2
1Departamento de Química, Centro de Investigación en Síntesis Química, Universidad de La Rioja, 26006 Logroño, La Rioja, Spain.
Abstract:
A self-immolative bioorthogonal conditionally cleavable linker based on Grob fragmentation is described. It is derived from 1,3-aminocyclohexanols and allows the release of sulfonate-containing compounds in aqueous media. Modulation of the amine pKa promotes fragmentation even at slightly acidic pH, a common feature of several tumor environments. The Grob fragmentation can also occur under physiological conditions in living cells, highlighting the potential bioorthogonal applicability of this reaction.
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