Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Evolutionary Relationships through Genome Comparisons

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Species delimitation in a cryptic Neotropical <i>Thelephora</i> species complex, with insights into the phylogenetic informativeness of loci.

Mycologia·2026

Same author

Do stomatal traits modulate leaf microbiome assembly?

The New phytologist·2026

Same author

Stomatal traits covary with leaf mycobiome diversity and composition.

The New phytologist·2025

Same author

Needle length in pines as a key trait regulating hydraulic resistance.

Annals of botany·2025

Same author

If You're Rare, Should I Care? How Imperfect Detection Changes Relationships Between Biodiversity and Global Change Drivers.

Global change biology·2025

Same author

Foliar mycobiome remains unaltered under urban air-pollution but differentially express stress-related genes.

Microbial ecology·2024

Same journal

A case of budding speciation in the California Floristic Province and infraspecific divergence in Abronia villosa.

American journal of botany·2026

Same journal

Soil salinity effects on pollen and pollinator visitation in a buzz-pollinated glycophyte, Solanum carolinense.

American journal of botany·2026

Same journal

Species-specific thermal thresholds for postdispersal embryo growth in Apiaceae.

American journal of botany·2026

Same journal

Taxonomic reassessment of fossil Sequoia and Protosequoia from the Upper Miocene of Central Honshu, Japan, with implications for leaf morphological variation in extant S. sempervirens.

American journal of botany·2026

Same journal

Biomechanics and anatomical investigation on the nodes and internodes of an arborescent monocotyledon.

American journal of botany·2026

Same journal

Erratum to "Phylogenomics resolves the evolutionary histories and taxonomy of the long-associated North American legume genera Psorodendron and Psorothamnus".

American journal of botany·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Oct 15, 2025

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Published on: May 2, 2018

Deep sequencing across multiple host species tests pine-endophyte specificity.

Jake Sarver¹, Ella Schultz¹, Austen Apigo¹

¹Department of Ecology, Evolution, and Marine Biology, University of California-Santa Barbara, Santa Barbara, California, 93106, USA.

American Journal of Botany

|October 25, 2021

Summary

This summary is machine-generated.

High-throughput sequencing rapidly identified fungal endophytes in pine species, but did not confirm host specialization patterns. This suggests current methods may overestimate fungal host specificity.

Keywords:

ITS1 Illumina MiSeq Sanger sequencing distance-decay internal transcribed spacer 1 phylogenetic specificity phylosymbiosis

More Related Videos

A Workflow for the Quantitative Assessment of the Endophytic and Epiphytic Bacterial Microbiomes of the Bark of Populus trichocarpa

A Workflow for the Quantitative Assessment of the Endophytic and Epiphytic Bacterial Microbiomes of the Bark of Populus trichocarpa

Published on: June 27, 2025

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants

Published on: May 5, 2023

Related Experiment Videos

Last Updated: Oct 15, 2025

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Published on: May 2, 2018

A Workflow for the Quantitative Assessment of the Endophytic and Epiphytic Bacterial Microbiomes of the Bark of Populus trichocarpa

A Workflow for the Quantitative Assessment of the Endophytic and Epiphytic Bacterial Microbiomes of the Bark of Populus trichocarpa

Published on: June 27, 2025

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants

Published on: May 5, 2023

Area of Science:

Ecology
Mycology
Molecular Biology

Background:

Foliar fungal endophytes exhibit varied distributions, suggesting specialized ecologies and host adaptations.
Understanding endophyte specialization requires broad host sampling, increasingly feasible with high-throughput sequencing.

Purpose of the Study:

To assess host specialization of the endophytic genus Lophodermium in six co-occurring pine species.
To evaluate the efficacy of amplicon sequencing for identifying fungal endophyte communities and host specificity patterns.

Main Methods:

Utilized amplicon sequencing to identify fungal endophytic communities in six pine (Pinus) species.
Focused on the genus Lophodermium to test for site and host specialization patterns.

Main Results:

Amplicon sequencing analyzed more samples and recovered more unique Lophodermium taxa than traditional culturing.
Patterns of host specialization were not better supported by amplicon sequencing compared to culturing methods.

Conclusions:

Amplicon sequencing enables rapid, large-scale surveys for identifying potential host specificity patterns.
Limitations exist in quantifying the intimacy of endophytic relationships using amplicon sequencing.