Reduction of multiple antibiotics from the waste water using coated glutathione S-transferase producing biocatalyst

Oxytetracycline is widely used in veterinary and human medicine. It has been detected in wastewater from pharmaceuticals, hospitals and domestic wastewater. In recent years, much more attention has been directed towards glutathione transferases (GSTs) because of their bio-transforming ability of antibiotics. In this study, 19 Lactobacillus strains were initially screened for the production of GSTs and five strains were selected for biotransformation of oxytetracycline. Among the strains, L. fermentum LA6 improved oxytetracyline degradation than other strains. It was subjected to optimize GST production and optimum growth was achieved after 24 h incubation at 32 ± 2 °C and 200 mg/L initial oxytetracycline concentration. The biocatalyst was immobilized and antibiotic degradation efficiency was analyzed. The immobilized culture of L. fermentum LA6 improved biodegradation of oxytetracycline in the wastewater. At 50 mg/L initial antibiotic concentration, 53.2 ± 2.8% oxytetracycline degradation was achieved, however, it improved at 200 mg/L antibiotic concentration in the culture medium (89.1 ± 4.3%) after 24 h. The chemical oxygen demand (COD) of the wastewater decreased significantly after treatment. At 200 mg/L oxytetracycline concentration, COD removal was considerably high (93.6 ± 5.3 mg/L) than 150 mg/L oxytetracycline concentration in the medium. Antibiotic removal efficiency in immobilized form revealed that this method is highly suitable for the removal of antibiotics from the wastewater.