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Updated: Oct 15, 2025
08:51
Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
Published on: March 15, 2019
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Nucleotide Pool Imbalance and Antibody Gene Diversification
Asim Azhar1, Nasim A Begum2, Afzal Husain3
1Department of Biotechnology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, India.
Vaccines
|October 26, 2021
Summary
Maintaining an optimal balance of deoxyribonucleoside triphosphates (dNTPs) is crucial for DNA polymerase function and genomic stability. This review explores how dNTP pool balance impacts antibody gene diversification through various DNA recombination and mutation processes.
Area of Science:
- Molecular Biology
- Genetics
- Immunology
Background:
- Deoxyribonucleoside triphosphate (dNTP) availability and balance are critical for DNA polymerase fidelity and processivity.
- Optimal dNTP pool maintenance is essential for genomic stability in both replicating and quiescent cells.
- Abnormalities in dNTP pools can affect DNA replication, repair, and recombination, impacting chromosomal activities.
Purpose of the Study:
- To review the role of optimal dNTP pool balance in antibody gene diversification.
- To discuss how dNTP pool imbalances affect DNA polymerases involved in immune system processes.
Main Methods:
- Literature review focused on dNTP pool dynamics and DNA metabolism.
- Analysis of the impact of dNTP levels on DNA polymerases.
- Examination of recombination and mutation processes in antibody gene generation.
Main Results:
- The dNTP pool significantly influences DNA polymerase activity, affecting DNA synthesis and repair fidelity.
- Imbalances in dNTP pools can lead to errors in DNA replication and recombination.
- Specific DNA polymerases involved in antibody diversification are sensitive to dNTP pool fluctuations.
Conclusions:
- Maintaining an optimal dNTP pool balance is vital for accurate DNA synthesis and genomic integrity.
- Proper dNTP levels are essential for the antigen-independent and antigen-dependent diversification of antibody encoding genes.
- Understanding dNTP pool regulation provides insights into immune system function and potential therapeutic targets.
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