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Establishment and Characterisation of Metastatic Extraskeletal Ewing Sarcoma Mouse Models.
Carmelo Cerra1, Michael A Harris1, Christine J Hawkins2
1Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, Australia.
In Vivo (Athens, Greece)
|October 26, 2021
Summary
New pre-clinical models effectively replicate metastatic extraskeletal Ewing sarcoma (ES) in mice, showing widespread spread and increased immune cell infiltration in metastases, aiding future therapy evaluation.
Area of Science:
- Oncology
- Translational Research
- Animal Models
Background:
- Ewing sarcoma typically originates in bone but can occur in soft tissues (extreskeletal).
- Extraskeletal Ewing sarcoma exhibits diverse metastatic patterns and poorer survival rates compared to skeletal forms.
- Existing animal models inadequately represent the metastatic complexity of extraskeletal Ewing sarcoma.
Purpose of the Study:
- To develop and validate pre-clinical models for extraskeletal Ewing sarcoma (ES).
- To accurately mimic the widespread metastatic potential of ES.
- To facilitate the study of ES disease progression and therapeutic strategies.
Main Methods:
- Luciferase-expressing ES cells from a muscle tumor were utilized.
- Cells were administered to nude mice via intramuscular or intravenous injection.
- Metastatic spread and tumor cellular composition were analyzed.
Main Results:
- Both injection routes led to metastasis in multiple organs (lungs, liver, kidneys, brain).
- Metastatic tumors showed significantly higher immune cell infiltration than primary intramuscular tumors.
- These models successfully recapitulated diverse metastatic sites.
Conclusions:
- Developed pre-clinical models accurately represent metastatic extraskeletal Ewing sarcoma.
- These models are valuable tools for evaluating novel therapies.
- Further understanding of ES disease progression is facilitated by these models.
