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Updated: Oct 15, 2025
Inducing Apical Periodontitis in Mice
Published on: August 6, 2019
Expression of Interleukin-17, Tumor Necrosis Factor-Alpha, and Matrix Metalloproteinase-8 in Patients with Chronic
Darius Gleiznys1, Albertas Kriauciunas1, Julius Maminskas1
1Department of Dental and Maxillofacial Orthopedics, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania.
Abstract:
BACKGROUND The present study aimed to evaluate whether non-surgical treatment interferes with clinical parameters and local patterns of osteo-immunoinflammatory mediators (IL-17 and TNF-alpha) and matrix metalloproteinase-8 (MMP-8) that are found in peri-implant crevicular fluid (PICF) and biofilms during the progression of peri-implant mucositis. MATERIAL AND METHODS We selected 30 patients with peri-implant caused mucositis before (MP) and after treatment (TP) and 30 healthy people (HP) for the analysis of IL-17, TNF-alpha cytokine, and MMP-8 production in PICF and for analysis of colonization dynamics of periodontopathogenic bacteria in supra- and subgingival plaque samples. The levels of IL-17 and MMP-8 concentrations in samples were assayed by enzymatic immunosorbent assay (ELISA) and TNF-alpha levels were determined by enzyme amplified sensitivity immunoassay (EASIA) method in PICF. The micro-IDent test was used to detect 11 species of periodontopathogenic bacteria in subgingival biofilm. RESULTS We found significantly (P<0.001) higher levels of IL-17, TNF-alpha, and MMP-8 in the PICF of the MP and TP groups in comparison to the HP group. A significant association was found in MP associated with Parvimonas micra, as TNF-alpha in PICF was significantly higher (P=0.034) than in patients without Parvimonas micra. TNF-alpha levels in the samples of PICF showed a moderate correlation with clinical parameters, including plaque index (PI) (P=0.007) and MMP-8 levels (P=0.001), in the MP group. CONCLUSIONS Assessment of levels of inflammatory cytokines in PICF can aid in the identification of peri-implant mucositis, which can assist in early diagnosis, prevention, and treatment.
Insights
Non-surgical treatment did not alter inflammatory mediators like IL-17, TNF-alpha, and MMP-8 in peri-implant mucositis patients. Measuring these cytokines in peri-implant crevicular fluid aids early diagnosis and treatment.
Area of Science:
- Oral immunology
- Periodontology
- Biomarker research
Background:
- Peri-implant mucositis is an inflammatory condition affecting implants.
- Understanding inflammatory mediators in peri-implant crevicular fluid (PICF) is crucial for managing mucositis.
Purpose of the Study:
- To evaluate the impact of non-surgical treatment on clinical parameters and inflammatory mediators in PICF and biofilms.
- To assess levels of IL-17, TNF-alpha, and MMP-8 in peri-implant mucositis progression.
Main Methods:
- Analysis of IL-17, TNF-alpha, and MMP-8 in PICF from patients with peri-implant mucositis (before and after treatment) and healthy controls.
- Detection of periodontopathogenic bacteria in subgingival plaque using the micro-IDent test.
- Quantification of cytokines via ELISA and EASIA methods.
Main Results:
- Significantly higher levels of IL-17, TNF-alpha, and MMP-8 were observed in PICF of treated and untreated peri-implant mucositis patients compared to healthy controls.
- Elevated TNF-alpha in PICF was associated with Parvimonas micra colonization.
- TNF-alpha levels showed a moderate correlation with plaque index and MMP-8 levels in the peri-implant mucositis group.
Conclusions:
- Non-surgical treatment did not normalize elevated inflammatory mediator levels in peri-implant mucositis.
- Assessing inflammatory cytokine levels in PICF can aid in the early identification, prevention, and treatment of peri-implant mucositis.
