Colorectal Carcinoma Growth Inhibition by Dietary Care Combined with Probiotic Intervention through Targeting NRP2...

The present study investigated the effect of probiotics on inhibition of colorectal tumor growth in vivo and as anti-proliferative agent in vitro. Viability changes were measured by MTT assay whereas protein expression was assessed using western blotting. The study demonstrated that tumor growth was delayed significantly (P < 0.05) in probiotic administered mice from 2nd week compared to the control group. The difference in body weight of the mice in probiotic administered, 5-fluorouracil treated and untreated groups of the mice showed no significant differences during 5-weeks of the study. In probiotic administered mice the expression of miR-331-3p was significantly promoted and that of NRP2 effectively alleviated. Probiotic administration of the mice led to a significant (P < 0.05) increase in p53 and p-c-Jun expression and reduction in Bcl-2 level. Probiotic treatment of SW480 and HCT116 cells led to a significant (P < 0.05) reduction in viability after 48 h compared to the control cells. However, no changes were observed in FHC cell viability after 48 h of treatment with probiotics. The expression of miR-331-3p in SW480 and HCT116 cells was significantly promoted on treatment with probiotics after 48 h. Additionally, probiotic treatment for 48 h led to a remarkable reduction in NRP2 expression in SW480 and HCT116 cells. Thus, probiotic administration inhibited colorectal tumor growth in vivo in mice possibly by upregulation of miR-331-3p expression and down-regulation of NRP2 level. Therefore, probiotics may be used for the treatment of colorectal cancer growth.