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Related Experiment Video

Updated: Oct 15, 2025

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

Published on: April 6, 2021

SARS-CoV-2 Multi-Antigen Serology Assay.

Ramin Mazhari^1,2, Shazia Ruybal-Pesántez^1,2,3, Fiona Angrisano³

¹Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3052, Australia.

Methods and Protocols

|October 26, 2021

Summary

This study details a new COVID-19 serological assay using Luminex bead technology. This method efficiently measures antibodies to SARS-CoV-2 in plasma and saliva for disease surveillance.

Keywords:

COVID-19 Luminex SARS-CoV-2 serology

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Area of Science:

Immunology
Virology
Biotechnology

Background:

Serology tests are crucial for identifying past pathogen exposure.
Measuring anti-SARS-CoV-2 antibodies is vital for understanding COVID-19 transmission dynamics.
High-throughput serological assays are needed for pandemic response.

Purpose of the Study:

To adapt Luminex bead-based technology for a COVID-19 multi-antigen serological assay.
To establish and optimize a protocol for measuring SARS-CoV-2 antibodies in human samples.
To provide a flexible assay for broad applicability in pathogen serosurveillance.

Main Methods:

Utilized Luminex bead-based technology for multiplexed antigen detection.
Developed and optimized a protocol for anti-SARS-CoV-2 antibody measurement.
Validated the assay for use with both plasma and saliva samples.

Main Results:

Successfully adapted Luminex technology for COVID-19 antibody detection.
Established a detailed protocol for assay optimization and implementation.
Demonstrated the assay's utility in measuring antibodies from different sample types.

Conclusions:

The developed Luminex-based assay is effective for high-throughput COVID-19 serology.
The protocol provides a robust method for SARS-CoV-2 antibody quantification.
This adaptable assay can be applied to study antibodies against various pathogens.