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Morphometric and Microstructural Changes During Murine Retinal Development Characterized Using In Vivo Optical

Simon Brais-Brunet1,2, Émilie Heckel2,3, Udayakumar Kanniyappan1,2

  • 1Institute of Biomedical Engineering, University of Montréal, Montréal, Canada.

Investigative Ophthalmology & Visual Science
|October 26, 2021
PubMed
Summary
This summary is machine-generated.

This study developed an in vivo optical coherence tomography (OCT) system to image mouse retinal development. The system tracked morphometric and microstructural changes, revealing key developmental mechanisms.

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Area of Science:

  • Ophthalmology
  • Developmental Biology
  • Biomedical Engineering

Background:

  • Mouse models are crucial for understanding retinal development and disease.
  • In vivo imaging techniques are essential for longitudinal studies of retinal maturation.
  • Optical Coherence Tomography (OCT) offers high-resolution cross-sectional imaging of retinal microstructure.

Purpose of the Study:

  • To develop an in vivo OCT system for imaging the developing mouse retina.
  • To characterize morphometric and microstructural changes during retinal development.
  • To establish a tool for studying developmental digressions in disease models.

Main Methods:

  • Developed a custom in vivo OCT system optimized for mouse retina imaging.
  • Imaged 34 wild-type mice from postnatal day 7 to P21.
  • Utilized semi-automatic segmentation to quantify retinal layer thicknesses (NFL, IPL, INL, ORL, total retina).

Main Results:

  • Significant IPL increase from P7-P10 (synaptogenesis) and P10-P12 (connectivity).
  • INL decreased significantly from P10-P12 (densification/pruning) and P17-P21 (thinning).
  • ORL increased from P10-P12 (photoreceptor maturation); NFL showed no time-dependent changes.

Conclusions:

  • Retinal layer thickness changes align with known developmental mechanisms.
  • The developed OCT system enables longitudinal characterization of mouse retinal development.
  • This system can be applied to study developmental abnormalities in models of human retinal diseases.